Comparison of quantitative carotenoid analysis using UV-Vis spectrophotometry and high-performance liquid chromatography
The quantification of β-carotene in agricultural by-products is crucial for their valorization. However, commonly used analytical methods, such as UV–Vis spectrophotometry and high-performance liquid chromatography (HPLC), present critical differences in specificity and cost. This study compared β-c...
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2026
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| author | Alfredo Arroyo Sánchez Noemí León-Roque Ancelmo Castillo Valdiviezo Thalia Alexandra Rivera-Ashqui Reynaldo J. Silva-Paz |
| author_browse | Alfredo Arroyo Sánchez Ancelmo Castillo Valdiviezo Noemí León-Roque Reynaldo J. Silva-Paz Thalia Alexandra Rivera-Ashqui |
| author_facet | Alfredo Arroyo Sánchez Noemí León-Roque Ancelmo Castillo Valdiviezo Thalia Alexandra Rivera-Ashqui Reynaldo J. Silva-Paz |
| author_sort | Alfredo Arroyo Sánchez |
| collection | Producción científica |
| description | The quantification of β-carotene in agricultural by-products is crucial for their valorization. However, commonly used analytical methods, such as UV–Vis spectrophotometry and high-performance liquid chromatography (HPLC), present critical differences in specificity and cost. This study compared β-carotene quantification using both methods, employing ethanol and hexane as extraction solvents, to determine β-carotene in peels from four mango varieties (Kent, Edward, Haden, and Criollo), following a 2×2×4 factorial design. Proximate analysis revealed significant differences in composition among the varieties. The results demonstrated a significant effect of the triple interaction (method × solvent × variety), indicating that all factors must be considered when selecting a β-carotene quantification protocol. Spectrophotometry systematically overestimated β-carotene content (up to 60% higher with ethanol) due to interference from other pigments. In contrast, HPLC provided specific quantification, revealing that hexane was significantly more efficient than ethanol for extraction. The Kent variety showed the highest β-carotene content. It is concluded that the HPLC–hexane combination is the optimal protocol for accurate quantification, whereas spectrophotometry provides only a useful screening estimate that should be interpreted with caution. The use of HPLC is recommended for studies requiring high accuracy, as well as for the validation of rapid methods against this standard for each new matrix. |
| format | Article |
| id | openalex:W7165140857 |
| institution | UPeU |
| language | English |
| publishDate | 2026 |
| publishDateRange | 2026 |
| publishDateSort | 2026 |
| record_format | dspace |
| spelling | openalex:W7165140857Comparison of quantitative carotenoid analysis using UV-Vis spectrophotometry and high-performance liquid chromatographyAlfredo Arroyo SánchezNoemí León-RoqueAncelmo Castillo ValdiviezoThalia Alexandra Rivera-AshquiReynaldo J. Silva-Pazmango peelmango cultivarsUV-Vis spectroscopyHPLCextraction solventsethanoln-hexanecarotenoid analysisagricultural by-productsmethod comparisonFood Nutrient Composition and Product DevelopmentPhytochemicals and Antioxidant ActivitiesAntioxidant Activity and Oxidative StressThe quantification of β-carotene in agricultural by-products is crucial for their valorization. However, commonly used analytical methods, such as UV–Vis spectrophotometry and high-performance liquid chromatography (HPLC), present critical differences in specificity and cost. This study compared β-carotene quantification using both methods, employing ethanol and hexane as extraction solvents, to determine β-carotene in peels from four mango varieties (Kent, Edward, Haden, and Criollo), following a 2×2×4 factorial design. Proximate analysis revealed significant differences in composition among the varieties. The results demonstrated a significant effect of the triple interaction (method × solvent × variety), indicating that all factors must be considered when selecting a β-carotene quantification protocol. Spectrophotometry systematically overestimated β-carotene content (up to 60% higher with ethanol) due to interference from other pigments. In contrast, HPLC provided specific quantification, revealing that hexane was significantly more efficient than ethanol for extraction. The Kent variety showed the highest β-carotene content. It is concluded that the HPLC–hexane combination is the optimal protocol for accurate quantification, whereas spectrophotometry provides only a useful screening estimate that should be interpreted with caution. The use of HPLC is recommended for studies requiring high accuracy, as well as for the validation of rapid methods against this standard for each new matrix.Articlehttps://doi.org/10.14295/cs.v17.4386https://comunicata.emnuvens.com.br/comunicata/article/download/4386/1418W7165140857enComunicata Scientiae2176-90792177-51332026-05-30 |
| spellingShingle | mango peel mango cultivars UV-Vis spectroscopy HPLC extraction solvents ethanol n-hexane carotenoid analysis agricultural by-products method comparison Food Nutrient Composition and Product Development Phytochemicals and Antioxidant Activities Antioxidant Activity and Oxidative Stress Alfredo Arroyo Sánchez Noemí León-Roque Ancelmo Castillo Valdiviezo Thalia Alexandra Rivera-Ashqui Reynaldo J. Silva-Paz Comparison of quantitative carotenoid analysis using UV-Vis spectrophotometry and high-performance liquid chromatography |
| title | Comparison of quantitative carotenoid analysis using UV-Vis spectrophotometry and high-performance liquid chromatography |
| title_full | Comparison of quantitative carotenoid analysis using UV-Vis spectrophotometry and high-performance liquid chromatography |
| title_fullStr | Comparison of quantitative carotenoid analysis using UV-Vis spectrophotometry and high-performance liquid chromatography |
| title_full_unstemmed | Comparison of quantitative carotenoid analysis using UV-Vis spectrophotometry and high-performance liquid chromatography |
| title_short | Comparison of quantitative carotenoid analysis using UV-Vis spectrophotometry and high-performance liquid chromatography |
| title_sort | comparison of quantitative carotenoid analysis using uv vis spectrophotometry and high performance liquid chromatography |
| topic | mango peel mango cultivars UV-Vis spectroscopy HPLC extraction solvents ethanol n-hexane carotenoid analysis agricultural by-products method comparison Food Nutrient Composition and Product Development Phytochemicals and Antioxidant Activities Antioxidant Activity and Oxidative Stress |
| topic_facet | mango peel mango cultivars UV-Vis spectroscopy HPLC extraction solvents ethanol n-hexane carotenoid analysis agricultural by-products method comparison Food Nutrient Composition and Product Development Phytochemicals and Antioxidant Activities Antioxidant Activity and Oxidative Stress |
| url | https://doi.org/10.14295/cs.v17.4386 https://comunicata.emnuvens.com.br/comunicata/article/download/4386/1418 |
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