Kinetic Modeling of the Growth of Saccharomyces cerevisiae in a Black Chuño (Freeze-Dried Potato)-Based Medium

Predictive microbiology enables the anticipation of microbial population behavior through mathematical models, a key tool in the development of fermented and functional foods. Black chuño, a traditional freeze-dried potato from the Andean region of Peru and Bolivia, is a substrate of cultural and nu...

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Autores principales: Nixon Arenas-Huahuachampi, Alex Danny Chambi-Rodríguez, Ana Mónica Torres Jiménez
Formato: Artículo
Lenguaje:inglés
Publicado: 2026
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Sumario:Predictive microbiology enables the anticipation of microbial population behavior through mathematical models, a key tool in the development of fermented and functional foods. Black chuño, a traditional freeze-dried potato from the Andean region of Peru and Bolivia, is a substrate of cultural and nutritional value whose potential as a culture medium remains underexplored. The objective of this study was to evaluate the growth kinetics of Saccharomyces cerevisiae in a black chuño-based medium. Lyophilized strains were activated in a 2% sucrose solution and inoculated into the medium at 37±1 °C, with constant agitation for 7 hours. Microbial growth was measured by direct counting in a Neubauer chamber, expressed in cfu/ml and transformed to a logarithmic scale; the data were fitted to nonlinear models: Gompertz, Logistic, Log-logistic, and Weibull. Growth was sigmoidal, with differences in the latency and maximum rate parameters. The log-logistic model showed the best fit, with biomass reaching a maximum of 0.663 log cfu/ml at hour 7. The study confirms the viability of black chuño as a culture medium and highlights the usefulness of mathematical models in microbial kinetics.