Determination of the diuretic effect of isaño (Tropaeolum tuberosum) and cola de caballo (Equisetum arvense) in mice
Isaño and cola de caballo are used in Bolivia for their traditional use as a diuretic, but there are nopharmacological studies that confirm this effect. Objective: to determine the diuretic effect of theaqueous extract of isaño and cola de caballo. Methodology: male mice were used, which were divide...
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| Publicado en: | Revista Científica de Ciencias de la Salud |
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Universidad Peruana Unión
2022
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| author | Castro Rua, Mabel Gutiérrez Patzi, Evelyn Pacheco Felipes, Gabriela Tola Guarachi, Elena Callisaya Mamani, Ana Flores Quispe, Mónica Gonzales Beltrán, Helen Villca Condori, André Apaza Chambi, Luz Terán Ventura, Evangelina |
| author_browse | Apaza Chambi, Luz Callisaya Mamani, Ana Castro Rua, Mabel Flores Quispe, Mónica Gonzales Beltrán, Helen Gutiérrez Patzi, Evelyn Pacheco Felipes, Gabriela Terán Ventura, Evangelina Tola Guarachi, Elena Villca Condori, André |
| author_facet | Castro Rua, Mabel Gutiérrez Patzi, Evelyn Pacheco Felipes, Gabriela Tola Guarachi, Elena Callisaya Mamani, Ana Flores Quispe, Mónica Gonzales Beltrán, Helen Villca Condori, André Apaza Chambi, Luz Terán Ventura, Evangelina |
| author_sort | Castro Rua, Mabel |
| collection | Revistas |
| container_title | Revista Científica de Ciencias de la Salud |
| description | Isaño and cola de caballo are used in Bolivia for their traditional use as a diuretic, but there are nopharmacological studies that confirm this effect. Objective: to determine the diuretic effect of theaqueous extract of isaño and cola de caballo. Methodology: male mice were used, which were dividedinto four groups, a positive and negative control and two dose groups (D1 and D2) of the extract. Bothdried plants were fractionated for the preparation of the aqueous extract, with which the preliminaryphytochemical screening was carried out, and the technique described by Lipschitz was carried out todetermine the diuretic effect. Results: The phytochemical analysis revealed the presence of flavonoidsfor the aqueous extract of cola de caballo. And the aqueous extract of Isaño showed the presenceof tannins, anthocyanins and flavonoids. The highest volume of urine collected was for the positivecontrol, and the lowest volume was for the negative control. The volumes of urine of the extract studiedwith respect to the negative and positive control are not statistically significant, but the value of thevolume of urine of the higher dose is closer to the value of the positive control. The electrolyte resultsshowed a decrease in the excretion of Na +, K + and Cl- in the urine of the problem mice with respectto the negative control group and the positive control, except the K + showed somewhat high valueswith respect to the positive control. Conclusion: The results confirm a possible diuretic effect of isañoand cola de caballo |
| format | Online |
| id | oai:rccs.upeu.edu.pe:article/1658 |
| institution | UPeU |
| issn | 2411-0094 2306-0603 |
| language | Spanish |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | Universidad Peruana Unión |
| publisherStr | Universidad Peruana Unión |
| record_format | ojs |
| revista_str_mv | Revista Científica de Ciencias de la Salud |
| spelling | oai:rccs.upeu.edu.pe:article/16582022-06-19T06:37:47Z Determination of the diuretic effect of isaño (Tropaeolum tuberosum) and cola de caballo (Equisetum arvense) in mice Determinación del efecto diurético del isaño (Tropaeolum tuberosum) y cola de caballo (Equisetum arvense) en ratones Castro Rua, Mabel Gutiérrez Patzi, Evelyn Pacheco Felipes, Gabriela Tola Guarachi, Elena Callisaya Mamani, Ana Flores Quispe, Mónica Gonzales Beltrán, Helen Villca Condori, André Apaza Chambi, Luz Terán Ventura, Evangelina Equisetum arvense, Tropaeolum tuberosum, diuretic effect, flavonoids, electrolytes, mice. Equisetum arvense, Tropaeolum tuberosum, efecto diurético, flavonoides, electrolitos, ratones. Isaño and cola de caballo are used in Bolivia for their traditional use as a diuretic, but there are nopharmacological studies that confirm this effect. Objective: to determine the diuretic effect of theaqueous extract of isaño and cola de caballo. Methodology: male mice were used, which were dividedinto four groups, a positive and negative control and two dose groups (D1 and D2) of the extract. Bothdried plants were fractionated for the preparation of the aqueous extract, with which the preliminaryphytochemical screening was carried out, and the technique described by Lipschitz was carried out todetermine the diuretic effect. Results: The phytochemical analysis revealed the presence of flavonoidsfor the aqueous extract of cola de caballo. And the aqueous extract of Isaño showed the presenceof tannins, anthocyanins and flavonoids. The highest volume of urine collected was for the positivecontrol, and the lowest volume was for the negative control. The volumes of urine of the extract studiedwith respect to the negative and positive control are not statistically significant, but the value of thevolume of urine of the higher dose is closer to the value of the positive control. The electrolyte resultsshowed a decrease in the excretion of Na +, K + and Cl- in the urine of the problem mice with respectto the negative control group and the positive control, except the K + showed somewhat high valueswith respect to the positive control. Conclusion: The results confirm a possible diuretic effect of isañoand cola de caballo El isaño y la cola de caballo son utilizados en Bolivia por su uso tradicional como diurético, pero nose tienen estudios farmacológicos que confirmen este efecto. Objetivo: determinar el efecto diuréticodel extracto acuoso de isaño y cola de caballo. Metodología: se utilizaron ratones macho, que sedividieron en cuatro grupos, un control positivo, negativo y dos grupos de dosis (D1 y D2) del extracto.Ambas plantas secas se fraccionaron para la preparación del extracto acuoso, con el que se realizóel tamizaje fitoquímico preliminar, y para determinar el efecto diurético se realizó la técnica descritapor Lipschitz. Resultados: La marcha fitoquímica reveló la presencia de flavonoides para el extractoacuoso de Cola de caballo. Y el extracto acuoso de Isaño mostró la presencia de taninos, antocianinasy flavonoides. El mayor volumen de orina recolectada fue para el control positivo, y el menor volumenfue para el control negativo. Los volúmenes de orina del extracto estudiado con respecto al controlnegativo y positivo estadísticamente no son significativos, pero el valor del volumen de orina de ladosis mayor se acerca más al valor del control positivo. Los resultados de electrolitos mostraron unadisminución en la excreción de Na+, K+ y Cl- en orina de los ratones problema con respecto al grupocontrol negativo y control positivo, excepto el K+ mostró valores algo elevados con respecto al controlpositivo. Conclusión: Los resultados confirman un posible efecto diurético del isaño y cola de caballo Universidad Peruana Unión 2022-01-31 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf https://rccs.upeu.edu.pe/index.php/rc_salud/article/view/1658 10.17162/rccs.v14i2.1658 Revista Científica de Ciencias de la Salud; Vol. 14 No. 2 (2021): Revista Científica de Ciencias de la Salud; 80 - 90 Revista Científica de Ciencias de la Salud; Vol. 14 Núm. 2 (2021): Revista Científica de Ciencias de la Salud; 80 - 90 2411-0094 2306-0603 10.17162/rccs.v14i2 spa https://rccs.upeu.edu.pe/index.php/rc_salud/article/view/1658/1937 Derechos de autor 2022 Mabel Castro Rua, Evelyn Gutiérrez Patzi, Gabriela Pacheco Felipes, Elena Tola Guarachi, Ana Callisaya Mamani, Mónica Flores Quispe, Helen Gonzales Beltrán, André Villca Condori, Luz Apaza Chambi, Evangelina Terán Ventura |
| spellingShingle | Equisetum arvense, Tropaeolum tuberosum, diuretic effect, flavonoids, electrolytes, mice. Equisetum arvense, Tropaeolum tuberosum, efecto diurético, flavonoides, electrolitos, ratones. Castro Rua, Mabel Gutiérrez Patzi, Evelyn Pacheco Felipes, Gabriela Tola Guarachi, Elena Callisaya Mamani, Ana Flores Quispe, Mónica Gonzales Beltrán, Helen Villca Condori, André Apaza Chambi, Luz Terán Ventura, Evangelina Determination of the diuretic effect of isaño (Tropaeolum tuberosum) and cola de caballo (Equisetum arvense) in mice |
| title | Determination of the diuretic effect of isaño (Tropaeolum tuberosum) and cola de caballo (Equisetum arvense) in mice |
| title_alt | Determinación del efecto diurético del isaño (Tropaeolum tuberosum) y cola de caballo (Equisetum arvense) en ratones |
| title_full | Determination of the diuretic effect of isaño (Tropaeolum tuberosum) and cola de caballo (Equisetum arvense) in mice |
| title_fullStr | Determination of the diuretic effect of isaño (Tropaeolum tuberosum) and cola de caballo (Equisetum arvense) in mice |
| title_full_unstemmed | Determination of the diuretic effect of isaño (Tropaeolum tuberosum) and cola de caballo (Equisetum arvense) in mice |
| title_short | Determination of the diuretic effect of isaño (Tropaeolum tuberosum) and cola de caballo (Equisetum arvense) in mice |
| title_sort | determination of the diuretic effect of isano tropaeolum tuberosum and cola de caballo equisetum arvense in mice |
| topic | Equisetum arvense, Tropaeolum tuberosum, diuretic effect, flavonoids, electrolytes, mice. Equisetum arvense, Tropaeolum tuberosum, efecto diurético, flavonoides, electrolitos, ratones. |
| topic_facet | Equisetum arvense, Tropaeolum tuberosum, diuretic effect, flavonoids, electrolytes, mice. Equisetum arvense, Tropaeolum tuberosum, efecto diurético, flavonoides, electrolitos, ratones. |
| url | https://rccs.upeu.edu.pe/index.php/rc_salud/article/view/1658 |
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