Musca domestica as a vector of colistin-resistant bacteria from dumpsites at two markets in Santa Anita, Lima
Introduction: Musca domestica can carry antimicrobial-resistant bacteria. Objectives: To evaluate its role as a vector of bacteria with phenotypic and genotypic resistance to colistin associated with the mcr-1 gene. Methods: A descriptive study was conducted between February and July 2023 at two mar...
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2026
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| author | Miguel A. Alarcón-Calle María Elena Rodrigo Rojas Ramsés Salas Asencios José Yareta-Yareta Pool Marcos-Carbajal |
| author_browse | José Yareta-Yareta María Elena Rodrigo Rojas Miguel A. Alarcón-Calle Pool Marcos-Carbajal Ramsés Salas Asencios |
| author_facet | Miguel A. Alarcón-Calle María Elena Rodrigo Rojas Ramsés Salas Asencios José Yareta-Yareta Pool Marcos-Carbajal |
| author_sort | Miguel A. Alarcón-Calle |
| collection | Producción científica |
| description | Introduction: Musca domestica can carry antimicrobial-resistant bacteria. Objectives: To evaluate its role as a vector of bacteria with phenotypic and genotypic resistance to colistin associated with the mcr-1 gene. Methods: A descriptive study was conducted between February and July 2023 at two market dumpsites in Santa Anita, Lima, Peru. A total of 60 flies were collected using adhesive plates baited with chicken. Samples were enriched in Tryptic Soy Broth and cultured on MacConkey agar. Bacterial identification was performed using conventional biochemical tests and MicroScan® for non-Enterobacteriaceae bacteria. Colistin resistance was assessed using the disk elution method in cation-adjusted Mueller–Hinton broth. Bacterial DNA was extracted using a commercial kit, and the mcr-1 gene was detected by conventional PCR. Results: Seventy-four bacterial isolates belonging to eight genera and 12 species were recovered. Escherichia coli was the most frequent species, accounting for 24.3% of isolates, followed by Klebsiella oxytoca, Proteus mirabilis, Klebsiella pneumoniae, and Klebsiella aerogenes. Phenotypic resistance to colistin was identified in 79.7% of isolates, with a minimum inhibitory concentration (MIC) ≥4 µg/mL. The mcr-1 gene was detected in four isolates of Klebsiella oxytoca, Klebsiella pneumoniae, and Escherichia coli. The association between phenotypic resistance and detection of the mcr-1 gene was significant, with a p-value of 0.035. Conclusion: Musca domestica may act as a mechanical carrier of colistin-resistant bacteria in urban dumpsites; therefore, its surveillance should be integrated into antimicrobial resistance control programs. Keywords: Houseflies; drug resistance, bacterial; colistin; Escherichia coli; Klebsiella pneumoniae (source: MeSH NLM) |
| format | Article |
| id | openalex:W7172138390 |
| institution | UPeU |
| language | English |
| publishDate | 2026 |
| publishDateRange | 2026 |
| publishDateSort | 2026 |
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| spelling | openalex:W7172138390Musca domestica as a vector of colistin-resistant bacteria from dumpsites at two markets in Santa Anita, LimaMiguel A. Alarcón-CalleMaría Elena Rodrigo RojasRamsés Salas AsenciosJosé Yareta-YaretaPool Marcos-CarbajalMusca domesticacolistin resistancemcr-1antimicrobial resistancevector-borne transmissionwaste disposal sitesfood marketsEscherichia coliKlebsiella pneumoniaeKlebsiella oxytocaProteus mirabilisurban sanitationSanta Anita, LimaAntibiotic Resistance in BacteriaInsects and Parasite InteractionsSalmonella and Campylobacter epidemiologyIntroduction: Musca domestica can carry antimicrobial-resistant bacteria. Objectives: To evaluate its role as a vector of bacteria with phenotypic and genotypic resistance to colistin associated with the mcr-1 gene. Methods: A descriptive study was conducted between February and July 2023 at two market dumpsites in Santa Anita, Lima, Peru. A total of 60 flies were collected using adhesive plates baited with chicken. Samples were enriched in Tryptic Soy Broth and cultured on MacConkey agar. Bacterial identification was performed using conventional biochemical tests and MicroScan® for non-Enterobacteriaceae bacteria. Colistin resistance was assessed using the disk elution method in cation-adjusted Mueller–Hinton broth. Bacterial DNA was extracted using a commercial kit, and the mcr-1 gene was detected by conventional PCR. Results: Seventy-four bacterial isolates belonging to eight genera and 12 species were recovered. Escherichia coli was the most frequent species, accounting for 24.3% of isolates, followed by Klebsiella oxytoca, Proteus mirabilis, Klebsiella pneumoniae, and Klebsiella aerogenes. Phenotypic resistance to colistin was identified in 79.7% of isolates, with a minimum inhibitory concentration (MIC) ≥4 µg/mL. The mcr-1 gene was detected in four isolates of Klebsiella oxytoca, Klebsiella pneumoniae, and Escherichia coli. The association between phenotypic resistance and detection of the mcr-1 gene was significant, with a p-value of 0.035. Conclusion: Musca domestica may act as a mechanical carrier of colistin-resistant bacteria in urban dumpsites; therefore, its surveillance should be integrated into antimicrobial resistance control programs. Keywords: Houseflies; drug resistance, bacterial; colistin; Escherichia coli; Klebsiella pneumoniae (source: MeSH NLM)Articlehttps://doi.org/10.25176/rfmh.v26i2.8035W7172138390enRevista de la Facultad de Medicina Humana1814-54692308-05312026-06-30 |
| spellingShingle | Musca domestica colistin resistance mcr-1 antimicrobial resistance vector-borne transmission waste disposal sites food markets Escherichia coli Klebsiella pneumoniae Klebsiella oxytoca Proteus mirabilis urban sanitation Santa Anita, Lima Antibiotic Resistance in Bacteria Insects and Parasite Interactions Salmonella and Campylobacter epidemiology Miguel A. Alarcón-Calle María Elena Rodrigo Rojas Ramsés Salas Asencios José Yareta-Yareta Pool Marcos-Carbajal Musca domestica as a vector of colistin-resistant bacteria from dumpsites at two markets in Santa Anita, Lima |
| title | Musca domestica as a vector of colistin-resistant bacteria from dumpsites at two markets in Santa Anita, Lima |
| title_full | Musca domestica as a vector of colistin-resistant bacteria from dumpsites at two markets in Santa Anita, Lima |
| title_fullStr | Musca domestica as a vector of colistin-resistant bacteria from dumpsites at two markets in Santa Anita, Lima |
| title_full_unstemmed | Musca domestica as a vector of colistin-resistant bacteria from dumpsites at two markets in Santa Anita, Lima |
| title_short | Musca domestica as a vector of colistin-resistant bacteria from dumpsites at two markets in Santa Anita, Lima |
| title_sort | musca domestica as a vector of colistin resistant bacteria from dumpsites at two markets in santa anita lima |
| topic | Musca domestica colistin resistance mcr-1 antimicrobial resistance vector-borne transmission waste disposal sites food markets Escherichia coli Klebsiella pneumoniae Klebsiella oxytoca Proteus mirabilis urban sanitation Santa Anita, Lima Antibiotic Resistance in Bacteria Insects and Parasite Interactions Salmonella and Campylobacter epidemiology |
| topic_facet | Musca domestica colistin resistance mcr-1 antimicrobial resistance vector-borne transmission waste disposal sites food markets Escherichia coli Klebsiella pneumoniae Klebsiella oxytoca Proteus mirabilis urban sanitation Santa Anita, Lima Antibiotic Resistance in Bacteria Insects and Parasite Interactions Salmonella and Campylobacter epidemiology |
| url | https://doi.org/10.25176/rfmh.v26i2.8035 |
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