Extraction, characterization and evaluation of antibacterial activity of essential oil of Senecio graveolens Wedd (Wiskataya)

The aim of this work was extract, characterize and evaluate the antibacterial activity of the essential oil of Senecio graveolens Wedd (Wiskataya) against Escherichia coli and Staphylococcus aureus ATCC 29923. The leaves and stems of S. graveolens were collected in the district of Puquio 3800 m.s.n....

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Autores principales: Kiev Ochoa Pumaylle, Luis Ricardo Paredes Quiroz, Dagnith Liz Bejarano Luján, Reynaldo J. Silva-Paz
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Publicado: 2012
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author Kiev Ochoa Pumaylle
Luis Ricardo Paredes Quiroz
Dagnith Liz Bejarano Luján
Reynaldo J. Silva-Paz
author_browse Dagnith Liz Bejarano Luján
Kiev Ochoa Pumaylle
Luis Ricardo Paredes Quiroz
Reynaldo J. Silva-Paz
author_facet Kiev Ochoa Pumaylle
Luis Ricardo Paredes Quiroz
Dagnith Liz Bejarano Luján
Reynaldo J. Silva-Paz
author_sort Kiev Ochoa Pumaylle
collection Producción científica
description The aim of this work was extract, characterize and evaluate the antibacterial activity of the essential oil of Senecio graveolens Wedd (Wiskataya) against Escherichia coli and Staphylococcus aureus ATCC 29923. The leaves and stems of S. graveolens were collected in the district of Puquio 3800 m.s.n.m., Lucanas province, and department of Ayacucho. The essential oil was obtained by steam water destilation dried leaves and stems with yield 1.26% (w/w) to which physical testing were performed. The chemical composition was evaluated by gas chromatography with mass detector (GC-MS). Antibacterial activity of S. graveolens oil was tested by agar diffusion method in wells against Gram positive strains such as S. aureus ATCC 29923 and Gram negative as E. coli. The density a 20 ºC was 0.8755 g/ml; the index refraction was 1.4726 and the rate rotation was 102° 85' and the solubility miscible in ethanol. The GC-MS showed the main components sabinene (52.39 %), (+)-4-carene (8.20 %), τ-terpinen (7.11 %), β-myrcene (6.74 %), 4-terpinenol (3.78 %) and pulegone (3.67 %). The results showing activity strong antibacterial activity and moderate, respectively, for the strains tested, observing formation of inhibition halos for essential oil concentrations at 80, 90 and 100 % in both strains. The essential oil of S. graveolens presented with promising antibacterial activity.
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spelling openalex:W1530542013Extraction, characterization and evaluation of antibacterial activity of essential oil of Senecio graveolens Wedd (Wiskataya)Kiev Ochoa PumaylleLuis Ricardo Paredes QuirozDagnith Liz Bejarano LujánReynaldo J. Silva-Pazessential oilsantibacterial activityEscherichia coliStaphylococcus aureuschemical compositionsabinenepulegoneterpenesmedicinal plantsAyacuchoEssential Oils and Antimicrobial ActivityInsect Pest Control StrategiesMedicinal Plant ResearchThe aim of this work was extract, characterize and evaluate the antibacterial activity of the essential oil of Senecio graveolens Wedd (Wiskataya) against Escherichia coli and Staphylococcus aureus ATCC 29923. The leaves and stems of S. graveolens were collected in the district of Puquio 3800 m.s.n.m., Lucanas province, and department of Ayacucho. The essential oil was obtained by steam water destilation dried leaves and stems with yield 1.26% (w/w) to which physical testing were performed. The chemical composition was evaluated by gas chromatography with mass detector (GC-MS). Antibacterial activity of S. graveolens oil was tested by agar diffusion method in wells against Gram positive strains such as S. aureus ATCC 29923 and Gram negative as E. coli. The density a 20 ºC was 0.8755 g/ml; the index refraction was 1.4726 and the rate rotation was 102° 85' and the solubility miscible in ethanol. The GC-MS showed the main components sabinene (52.39 %), (+)-4-carene (8.20 %), τ-terpinen (7.11 %), β-myrcene (6.74 %), 4-terpinenol (3.78 %) and pulegone (3.67 %). The results showing activity strong antibacterial activity and moderate, respectively, for the strains tested, observing formation of inhibition halos for essential oil concentrations at 80, 90 and 100 % in both strains. The essential oil of S. graveolens presented with promising antibacterial activity.Articlehttps://doi.org/10.17268/sci.agropecu.2012.04.03https://revistas.unitru.edu.pe/index.php/scientiaagrop/article/download/90/22W1530542013enScientia Agropecuaria2077-99172306-67412012-12-31
spellingShingle essential oils
antibacterial activity
Escherichia coli
Staphylococcus aureus
chemical composition
sabinene
pulegone
terpenes
medicinal plants
Ayacucho
Essential Oils and Antimicrobial Activity
Insect Pest Control Strategies
Medicinal Plant Research
Kiev Ochoa Pumaylle
Luis Ricardo Paredes Quiroz
Dagnith Liz Bejarano Luján
Reynaldo J. Silva-Paz
Extraction, characterization and evaluation of antibacterial activity of essential oil of Senecio graveolens Wedd (Wiskataya)
title Extraction, characterization and evaluation of antibacterial activity of essential oil of Senecio graveolens Wedd (Wiskataya)
title_full Extraction, characterization and evaluation of antibacterial activity of essential oil of Senecio graveolens Wedd (Wiskataya)
title_fullStr Extraction, characterization and evaluation of antibacterial activity of essential oil of Senecio graveolens Wedd (Wiskataya)
title_full_unstemmed Extraction, characterization and evaluation of antibacterial activity of essential oil of Senecio graveolens Wedd (Wiskataya)
title_short Extraction, characterization and evaluation of antibacterial activity of essential oil of Senecio graveolens Wedd (Wiskataya)
title_sort extraction characterization and evaluation of antibacterial activity of essential oil of senecio graveolens wedd wiskataya
topic essential oils
antibacterial activity
Escherichia coli
Staphylococcus aureus
chemical composition
sabinene
pulegone
terpenes
medicinal plants
Ayacucho
Essential Oils and Antimicrobial Activity
Insect Pest Control Strategies
Medicinal Plant Research
topic_facet essential oils
antibacterial activity
Escherichia coli
Staphylococcus aureus
chemical composition
sabinene
pulegone
terpenes
medicinal plants
Ayacucho
Essential Oils and Antimicrobial Activity
Insect Pest Control Strategies
Medicinal Plant Research
url https://doi.org/10.17268/sci.agropecu.2012.04.03
https://revistas.unitru.edu.pe/index.php/scientiaagrop/article/download/90/22
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AT dagnithlizbejaranolujan extractioncharacterizationandevaluationofantibacterialactivityofessentialoilofseneciograveolensweddwiskataya
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