Development of a Hybrid System Based on the CIELAB Colour Space and Artificial Neural Networks for Monitoring pH and Acidity During Yogurt Fermentation

Monitoring pH and acidity during yoghurt fermentation is essential for product quality and process efficiency. Conventional measurement methods, however, are invasive and labourintensive. This study developed artificial neural network (ANN) models to predict pH and titratable acidity during yoghurt...

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Autores principales: Alvarado, Ulises, Tacuriti, Juan, Coloma, Alejandro, Gallegos Rojas, Edgar, Callo, Herbert, Valencia-Sullca, Cristina, Rafael, Nancy Curasi, Castillo, Manuel
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Publicado: Multidisciplinary Digital Publishing Institute (MDPI) 2025
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author Alvarado, Ulises
Tacuriti, Juan
Coloma, Alejandro
Gallegos Rojas, Edgar
Callo, Herbert
Valencia-Sullca, Cristina
Rafael, Nancy Curasi
Castillo, Manuel
author_browse Alvarado, Ulises
Callo, Herbert
Castillo, Manuel
Coloma, Alejandro
Gallegos Rojas, Edgar
Rafael, Nancy Curasi
Tacuriti, Juan
Valencia-Sullca, Cristina
author_facet Alvarado, Ulises
Tacuriti, Juan
Coloma, Alejandro
Gallegos Rojas, Edgar
Callo, Herbert
Valencia-Sullca, Cristina
Rafael, Nancy Curasi
Castillo, Manuel
author_sort Alvarado, Ulises
collection Repositorio institucional
description Monitoring pH and acidity during yoghurt fermentation is essential for product quality and process efficiency. Conventional measurement methods, however, are invasive and labourintensive. This study developed artificial neural network (ANN) models to predict pH and titratable acidity during yoghurt fermentation using CIELAB colour parameters (L, a*, b*). Reconstituted milk powder with 12% total solids was prepared with varying protein levels (4.2–4.8%), inoculum concentrations (1–3%), and fermentation temperatures (36–44 ◦C). Data were collected every 10 min until pH 4.6 was reached. Forty models were trained for each output variable, using 90% of the data for training and 10% for validation. The first two phases of the fermentation process were clearly distinguishable, lasting between 4.5 and 7 h and exceeding 0.6% lactic acid in all treatments evaluated. The best pH model used two hidden layers with 28 neurons (R2 = 0.969; RMSE = 0.007), while the optimal acidity model had four hidden layers with 32 neurons (R2 = 0.868; RMSE = 0.002). The strong correlation between colour and physicochemical changes confirms the feasibility of this non-destructive approach. Integrating ANN models and colourimetry offers a practical solution for real-time monitoring, helping improve process control in industrial yoghurt production.
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language English
ocde_area_str_mv Ciencias médicas y de la salud
ocde_disciplina_str_mv Ciencias de la salud
ocde_str_mv 3.03.00
publishDate 2025
publishDateRange 2025
publishDateSort 2025
publisher Multidisciplinary Digital Publishing Institute (MDPI)
publisherStr Multidisciplinary Digital Publishing Institute (MDPI)
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spelling Development of a Hybrid System Based on the CIELAB Colour Space and Artificial Neural Networks for Monitoring pH and Acidity During Yogurt Fermentation Alvarado, Ulises Tacuriti, Juan Coloma, Alejandro Gallegos Rojas, Edgar Callo, Herbert Valencia-Sullca, Cristina Rafael, Nancy Curasi Castillo, Manuel Fermentation PH Acidity CIELAB colour space Artificial neural networks Monitoring pH and acidity during yoghurt fermentation is essential for product quality and process efficiency. Conventional measurement methods, however, are invasive and labourintensive. This study developed artificial neural network (ANN) models to predict pH and titratable acidity during yoghurt fermentation using CIELAB colour parameters (L, a*, b*). Reconstituted milk powder with 12% total solids was prepared with varying protein levels (4.2–4.8%), inoculum concentrations (1–3%), and fermentation temperatures (36–44 ◦C). Data were collected every 10 min until pH 4.6 was reached. Forty models were trained for each output variable, using 90% of the data for training and 10% for validation. The first two phases of the fermentation process were clearly distinguishable, lasting between 4.5 and 7 h and exceeding 0.6% lactic acid in all treatments evaluated. The best pH model used two hidden layers with 28 neurons (R2 = 0.969; RMSE = 0.007), while the optimal acidity model had four hidden layers with 32 neurons (R2 = 0.868; RMSE = 0.002). The strong correlation between colour and physicochemical changes confirms the feasibility of this non-destructive approach. Integrating ANN models and colourimetry offers a practical solution for real-time monitoring, helping improve process control in industrial yoghurt production. Consejo Nacional de Ciencia, Tecnología e Innovación Tecnológica Programa Nacional de Investigación Científica y Estudios Avanzados PE501082973-2023 2025-08-01 2026-03-10T20:08:05Z 2026-03-10T20:08:05Z info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion https://hdl.handle.net/20.500.12840/9883 https://doi.org/10.3390/dairy6040041 eng urn:issn:2624-862X info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ application/pdf Multidisciplinary Digital Publishing Institute (MDPI) PE oai:repositorio.upeu.edu.pe:20.500.12840/9883 2026-06-11T09:36:45Z
spellingShingle Fermentation
PH
Acidity
CIELAB colour space
Artificial neural networks
Alvarado, Ulises
Tacuriti, Juan
Coloma, Alejandro
Gallegos Rojas, Edgar
Callo, Herbert
Valencia-Sullca, Cristina
Rafael, Nancy Curasi
Castillo, Manuel
Development of a Hybrid System Based on the CIELAB Colour Space and Artificial Neural Networks for Monitoring pH and Acidity During Yogurt Fermentation
title Development of a Hybrid System Based on the CIELAB Colour Space and Artificial Neural Networks for Monitoring pH and Acidity During Yogurt Fermentation
title_full Development of a Hybrid System Based on the CIELAB Colour Space and Artificial Neural Networks for Monitoring pH and Acidity During Yogurt Fermentation
title_fullStr Development of a Hybrid System Based on the CIELAB Colour Space and Artificial Neural Networks for Monitoring pH and Acidity During Yogurt Fermentation
title_full_unstemmed Development of a Hybrid System Based on the CIELAB Colour Space and Artificial Neural Networks for Monitoring pH and Acidity During Yogurt Fermentation
title_short Development of a Hybrid System Based on the CIELAB Colour Space and Artificial Neural Networks for Monitoring pH and Acidity During Yogurt Fermentation
title_sort development of a hybrid system based on the cielab colour space and artificial neural networks for monitoring ph and acidity during yogurt fermentation
topic Fermentation
PH
Acidity
CIELAB colour space
Artificial neural networks
topic_facet Fermentation
PH
Acidity
CIELAB colour space
Artificial neural networks
url https://hdl.handle.net/20.500.12840/9883
https://doi.org/10.3390/dairy6040041
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