Isolation of Gram-negative bacilli and Staphylococci, after two handwashing techniques, in Nursing students who perform hospital practices
Objective: The purpose of the research was the isolation of microorganisms of the genusStaphylococcus and Bacillus Gram negative after two handwashing techniques, during the laboratorypractices of nursing students. Methodology: The research was correlational and transversal, carriedout in May 2018;...
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| Publicado en: | Revista Científica de Ciencias de la Salud |
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| Autores principales: | , , |
| Formato: | Artículo de revista |
| Lenguaje: | español |
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Universidad Peruana Unión
2019
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| _version_ | 1881695092184449024 |
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| author | Orellana Guevara, Raquel Colque Flores, Celia Yugar Mier, Max Abel |
| author_browse | Colque Flores, Celia Orellana Guevara, Raquel Yugar Mier, Max Abel |
| author_facet | Orellana Guevara, Raquel Colque Flores, Celia Yugar Mier, Max Abel |
| author_sort | Orellana Guevara, Raquel |
| collection | Revistas |
| container_title | Revista Científica de Ciencias de la Salud |
| description | Objective: The purpose of the research was the isolation of microorganisms of the genusStaphylococcus and Bacillus Gram negative after two handwashing techniques, during the laboratorypractices of nursing students. Methodology: The research was correlational and transversal, carriedout in May 2018; The participants were 40 students distributed in two groups (Control and experimentalgroups). Samples were taken by swab from recently washed hands. The 11-step clinical handwashing(CML) was applied in the control group and the 3-cycle CML in the experimental group, after training.Isolation, identification and microbial count was carried out in suitable and selective culture media.The data was processed in the Excel program, using tests of statistical significance and descriptivestatistics. Results: Negative Staphylococcus cuagulase was isolated in 40% of the total participants;
Depending on the type of washing this microorganism was found in 50% of participants who appliedthe 11-step CML, and in 30% of participants after the 3-stroke CML. Regarding the search for Gramnegativebacilli, Klebsiella spp was found in 5% of the total participants in the 3-stroke CML. Bacterialcounts were lower in the 3-stroke CML and a statistically significant difference of p = 0.033 (p≤0.05) wasobtained when comparing both washing techniques. Conclusions: There was a difference betweenthe two handwashing techniques in bacterial counts, with greater growth after the 11-step CML, andlower growth in the 3-stroke CML. The most frequent microorganism was Staphylococcus cuagulasenegative and in a minimum of samples, Klebsiella spp. |
| format | Online |
| id | oai:rccs.upeu.edu.pe:article/1218 |
| institution | UPeU |
| issn | 2411-0094 2306-0603 |
| language | Spanish |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | Universidad Peruana Unión |
| publisherStr | Universidad Peruana Unión |
| record_format | ojs |
| revista_str_mv | Revista Científica de Ciencias de la Salud |
| spelling | oai:rccs.upeu.edu.pe:article/12182020-02-25T17:35:09Z Isolation of Gram-negative bacilli and Staphylococci, after two handwashing techniques, in Nursing students who perform hospital practices Aislamiento de bacilos Gram negativos y Estafilococos, posterior a dos técnicas de lavado de manos, en estudiantes de Enfermería que realizan prácticas hospitalarias Orellana Guevara, Raquel Colque Flores, Celia Yugar Mier, Max Abel Handwashing, Staphylococcus, Gram negative bacilli Lavado de manos, Staphylococcus, Bacilos Gram negativos Objective: The purpose of the research was the isolation of microorganisms of the genusStaphylococcus and Bacillus Gram negative after two handwashing techniques, during the laboratorypractices of nursing students. Methodology: The research was correlational and transversal, carriedout in May 2018; The participants were 40 students distributed in two groups (Control and experimentalgroups). Samples were taken by swab from recently washed hands. The 11-step clinical handwashing(CML) was applied in the control group and the 3-cycle CML in the experimental group, after training.Isolation, identification and microbial count was carried out in suitable and selective culture media.The data was processed in the Excel program, using tests of statistical significance and descriptivestatistics. Results: Negative Staphylococcus cuagulase was isolated in 40% of the total participants; Depending on the type of washing this microorganism was found in 50% of participants who appliedthe 11-step CML, and in 30% of participants after the 3-stroke CML. Regarding the search for Gramnegativebacilli, Klebsiella spp was found in 5% of the total participants in the 3-stroke CML. Bacterialcounts were lower in the 3-stroke CML and a statistically significant difference of p = 0.033 (p≤0.05) wasobtained when comparing both washing techniques. Conclusions: There was a difference betweenthe two handwashing techniques in bacterial counts, with greater growth after the 11-step CML, andlower growth in the 3-stroke CML. The most frequent microorganism was Staphylococcus cuagulasenegative and in a minimum of samples, Klebsiella spp. Objetivo: El propósito de la investigación fue el aislamiento de microorganismos del géneroStaphylococcus y Bacilos Gram negativos posterior a dos técnicas de lavado de manos, durantelas prácticas de laboratorio de los estudiantes de Enfermería. Metodología: La investigación fue detipo correlacional y transversal, realizada en mayo de 2018; los participantes fueron 40 estudiantesdistribuidos en dos grupos (Grupos control y experimental). Las muestras se tomaron por hisopadode las manos recientemente lavadas. Se aplicó el lavado de manos clínico (LMC) de 11 pasos en elgrupo control y el LMC de 3 tiempos en el grupo experimental, previa capacitación. El aislamiento,identificación y recuento microbiano se efectuó en medios de cultivos adecuados y selectivos. Losdatos se procesaron en el programa Excel, mediante pruebas de significación estadística y estadísticosdescriptivos. Resultados: Se aisló Staphylococcus cuagulasa negativo en un 40 % del total departicipantes; según el tipo de lavado se encontró este microorganismo en un 50% de participantesque aplicaron el LMC de 11 pasos, y en un 30% de participantes tras el LMC de 3 tiempos. Respecto ala búsqueda de bacilos Gram negativos, se encontró Klebsiella spp en un 5% del total de participantesen el LMC de 3 tiempos. Los recuentos bacterianos fueron más bajos en el LMC de 3 tiempos y seobtuvo diferencia estadísticamente significativa de p=0.033 (p≤0.05) al comparar ambas técnicas delavado. Conclusiones: Hubo diferencia entre las dos técnicas de lavado de manos en los recuentosbacterianos, hallándose mayor crecimiento tras el LMC de 11 pasos, y menor crecimiento en el LMCde 3 tiempos. El microorganismo más frecuente fue Staphylococcus cuagulasa negativo y en unmínimo de muestras, Klebsiella spp. Universidad Peruana Unión 2019-12-02 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion application/pdf text/html https://rccs.upeu.edu.pe/index.php/rc_salud/article/view/1218 10.17162/rccs.v12i2.1218 Revista Científica de Ciencias de la Salud; Vol. 12 No. 2 (2019): Revista Científica de Ciencias de la Salud Revista Científica de Ciencias de la Salud; Vol. 12 Núm. 2 (2019): Revista Científica de Ciencias de la Salud 2411-0094 2306-0603 10.17162/rccs.v12i2 spa https://rccs.upeu.edu.pe/index.php/rc_salud/article/view/1218/1554 https://rccs.upeu.edu.pe/index.php/rc_salud/article/view/1218/1555 |
| spellingShingle | Handwashing, Staphylococcus, Gram negative bacilli Lavado de manos, Staphylococcus, Bacilos Gram negativos Orellana Guevara, Raquel Colque Flores, Celia Yugar Mier, Max Abel Isolation of Gram-negative bacilli and Staphylococci, after two handwashing techniques, in Nursing students who perform hospital practices |
| title | Isolation of Gram-negative bacilli and Staphylococci, after two handwashing techniques, in Nursing students who perform hospital practices |
| title_alt | Aislamiento de bacilos Gram negativos y Estafilococos, posterior a dos técnicas de lavado de manos, en estudiantes de Enfermería que realizan prácticas hospitalarias |
| title_full | Isolation of Gram-negative bacilli and Staphylococci, after two handwashing techniques, in Nursing students who perform hospital practices |
| title_fullStr | Isolation of Gram-negative bacilli and Staphylococci, after two handwashing techniques, in Nursing students who perform hospital practices |
| title_full_unstemmed | Isolation of Gram-negative bacilli and Staphylococci, after two handwashing techniques, in Nursing students who perform hospital practices |
| title_short | Isolation of Gram-negative bacilli and Staphylococci, after two handwashing techniques, in Nursing students who perform hospital practices |
| title_sort | isolation of gram negative bacilli and staphylococci after two handwashing techniques in nursing students who perform hospital practices |
| topic | Handwashing, Staphylococcus, Gram negative bacilli Lavado de manos, Staphylococcus, Bacilos Gram negativos |
| topic_facet | Handwashing, Staphylococcus, Gram negative bacilli Lavado de manos, Staphylococcus, Bacilos Gram negativos |
| url | https://rccs.upeu.edu.pe/index.php/rc_salud/article/view/1218 |
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