MEDIAN LETHAL CONCENTRATION (LC50) OF MERCURY CHLORIDE (HgCl2) TO “GAMITANA” FINGERLINGS Colossoma macropomum (CUVIER, 1818) IN CONTROLLED ENVIRONMENTS
In this study, the median lethal concentration (LC50) of mercury exposure at 96 hours in [Colossoma macropomum (Cuvier, 1818)] was estimated through static acute toxicity test by using mercury chloride (HgCl2) as a letal source. The experiment was carried out in a residence located in the district f...
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| Publicado en: | Revista de Investigación Ciencia, Tecnología y Desarrollo |
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Universidad Peruana Unión
2019
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| _version_ | 1881503195124989952 |
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| author | JAVIER OSCAR ZAVALETA FLORES ROBERTO PEZO DÍAZ |
| author_browse | JAVIER OSCAR ZAVALETA FLORES ROBERTO PEZO DÍAZ |
| author_facet | JAVIER OSCAR ZAVALETA FLORES ROBERTO PEZO DÍAZ |
| author_sort | JAVIER OSCAR ZAVALETA FLORES |
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| container_title | Revista de Investigación Ciencia, Tecnología y Desarrollo |
| description | In this study, the median lethal concentration (LC50) of mercury exposure at 96 hours in [Colossoma macropomum (Cuvier, 1818)] was estimated through static acute toxicity test by using mercury chloride (HgCl2) as a letal source. The experiment was carried out in a residence located in the district from San Juan Bautista, Province Maynas, Region Loreto (Peru), under controlled conditions (28.85 ± 0,15 °C) and a 12:12 (light: darkness) photoperiod. The gamitana fingerlings (4 ± 1 g) were housed in glass aquaria with constant aeration without filter and feeding was supressed 24 hours before beginning the experiment. Three concentrations of mercury (Hg) were used with replicas incluiding controls. The concentrations were: 0.01, 0.1 y 1 mg Hg/L. Histopathological analyses were conducted on 3 fish per treatment group on samples of gills tissues. Fish exposed to the lowest concentrations (0.01, 0.1 mg Hg/L) of HgCl2 showed hyperactivity, whereas fish exposed to high concentration (1 mg Hg/L) showed decreased activity. Histopathological analysis showed gills lesions, like a lamellar hiperplasia and lipid vacuolization, respectively, in response to detoxification processes. The value of LC50-96 h was estimated by using the TSK software (Trimmed-Spearman-Karber) and produced a value of 0.23 mg HgCl2/L ± 0.15.
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| format | Online |
| id | oai:ojs.pkp.sfu.ca:article/1352 |
| institution | UPeU |
| issn | 2410-843x 2313-7991 |
| language | Spanish |
| publishDate | 2019 |
| publishDateRange | 2019 |
| publishDateSort | 2019 |
| publisher | Universidad Peruana Unión |
| publisherStr | Universidad Peruana Unión |
| record_format | ojs |
| revista_str_mv | Revista de Investigación Ciencia, Tecnología y Desarrollo |
| spelling | oai:ojs.pkp.sfu.ca:article/13522020-10-05T16:06:08Z MEDIAN LETHAL CONCENTRATION (LC50) OF MERCURY CHLORIDE (HgCl2) TO “GAMITANA” FINGERLINGS Colossoma macropomum (CUVIER, 1818) IN CONTROLLED ENVIRONMENTS Concentración letal media (CL50) de cloruro de mercurio (HgCl2) sobre alevinos de “gamitana” Colossoma macropomum (Cuvier, 1818) en ambientes controlados JAVIER OSCAR ZAVALETA FLORES ROBERTO PEZO DÍAZ cloruro de mercurio, mercurio, toxicología acuática, Colossoma macropomum In this study, the median lethal concentration (LC50) of mercury exposure at 96 hours in [Colossoma macropomum (Cuvier, 1818)] was estimated through static acute toxicity test by using mercury chloride (HgCl2) as a letal source. The experiment was carried out in a residence located in the district from San Juan Bautista, Province Maynas, Region Loreto (Peru), under controlled conditions (28.85 ± 0,15 °C) and a 12:12 (light: darkness) photoperiod. The gamitana fingerlings (4 ± 1 g) were housed in glass aquaria with constant aeration without filter and feeding was supressed 24 hours before beginning the experiment. Three concentrations of mercury (Hg) were used with replicas incluiding controls. The concentrations were: 0.01, 0.1 y 1 mg Hg/L. Histopathological analyses were conducted on 3 fish per treatment group on samples of gills tissues. Fish exposed to the lowest concentrations (0.01, 0.1 mg Hg/L) of HgCl2 showed hyperactivity, whereas fish exposed to high concentration (1 mg Hg/L) showed decreased activity. Histopathological analysis showed gills lesions, like a lamellar hiperplasia and lipid vacuolization, respectively, in response to detoxification processes. The value of LC50-96 h was estimated by using the TSK software (Trimmed-Spearman-Karber) and produced a value of 0.23 mg HgCl2/L ± 0.15. En el presente se registra la concentración letal media (CL50) a 96 horas del mercurio en gamitana [Colossoma macropomum (Cuvier, 1818)]. Se calculó a través de una prueba estática de toxicidad aguda utilizando cloruro de mercurio (HgCl2) como agente letal. El experimento fue realizado el distrito de San Juan Bautista, Provincia de Maynas, Región Loreto (Perú), en condiciones controladas (28,85 ± 0,15 °C) y un fotoperiodo 12:12 (luz: oscuridad). Los alevinos (4 ± 1 g) fueron mantenidos en acuarios de vidrio con aireación constante, sin filtro y la alimentación fue suprimida 24 horas antes del inicio del experimento. Se emplearon 3 concentraciones de mercurio (Hg), con sus respectivas réplicas, incluyendo un grupo control. Las concentraciones fueron: 0,01, 0,1 y 1 mg Hg/L. Se realizó un análisis histopatológico con tres peces de cada tratamiento tomando muestras de branquias. Los especímenes expuestos a las concentraciones más bajas (0,01 y 0,1 mg Hg/L) mostraron hiperactividad, a diferencia de la concentración más alta (1 mg Hg/L) los cuales evidenciaron disminución de su actividad. El análisis histopatológico mostró anomalías en las branquias como hiperplasia interlamelar y vasculizaciones lipídicas respectivamente, en respuesta a procesos de detoxificación. El valor de la CL50-96 h fue estimado utilizando el programa TSK (Trimmed-Spearman-Karber) y presentó un valor de 0,23 mg HgCl2/L ± 0,15. Universidad Peruana Unión 2019-10-16 info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Artículo revisado por pares application/pdf https://revistas.upeu.edu.pe/ri_ctd/article/view/1352 10.17162/rictd.v5i1.1352 Revista de Investigación Ciencia, Tecnología y Desarrollo; Vol. 5 Núm. 1 (2019): Revista de Investigación: Ciencia, Tecnología y Desarrollo 2410-843x 2313-7991 10.17162/rictd.v5i1 spa https://revistas.upeu.edu.pe/ri_ctd/article/view/1352/1693 Derechos de autor 2019 Revista de Investigación Ciencia, Tecnología y Desarrollo |
| spellingShingle | cloruro de mercurio, mercurio, toxicología acuática, Colossoma macropomum JAVIER OSCAR ZAVALETA FLORES ROBERTO PEZO DÍAZ MEDIAN LETHAL CONCENTRATION (LC50) OF MERCURY CHLORIDE (HgCl2) TO “GAMITANA” FINGERLINGS Colossoma macropomum (CUVIER, 1818) IN CONTROLLED ENVIRONMENTS |
| title | MEDIAN LETHAL CONCENTRATION (LC50) OF MERCURY CHLORIDE (HgCl2) TO “GAMITANA” FINGERLINGS Colossoma macropomum (CUVIER, 1818) IN CONTROLLED ENVIRONMENTS |
| title_alt | Concentración letal media (CL50) de cloruro de mercurio (HgCl2) sobre alevinos de “gamitana” Colossoma macropomum (Cuvier, 1818) en ambientes controlados |
| title_full | MEDIAN LETHAL CONCENTRATION (LC50) OF MERCURY CHLORIDE (HgCl2) TO “GAMITANA” FINGERLINGS Colossoma macropomum (CUVIER, 1818) IN CONTROLLED ENVIRONMENTS |
| title_fullStr | MEDIAN LETHAL CONCENTRATION (LC50) OF MERCURY CHLORIDE (HgCl2) TO “GAMITANA” FINGERLINGS Colossoma macropomum (CUVIER, 1818) IN CONTROLLED ENVIRONMENTS |
| title_full_unstemmed | MEDIAN LETHAL CONCENTRATION (LC50) OF MERCURY CHLORIDE (HgCl2) TO “GAMITANA” FINGERLINGS Colossoma macropomum (CUVIER, 1818) IN CONTROLLED ENVIRONMENTS |
| title_short | MEDIAN LETHAL CONCENTRATION (LC50) OF MERCURY CHLORIDE (HgCl2) TO “GAMITANA” FINGERLINGS Colossoma macropomum (CUVIER, 1818) IN CONTROLLED ENVIRONMENTS |
| title_sort | median lethal concentration lc50 of mercury chloride hgcl2 to gamitana fingerlings colossoma macropomum cuvier 1818 in controlled environments |
| topic | cloruro de mercurio, mercurio, toxicología acuática, Colossoma macropomum |
| topic_facet | cloruro de mercurio, mercurio, toxicología acuática, Colossoma macropomum |
| url | https://revistas.upeu.edu.pe/ri_ctd/article/view/1352 |
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