IFN-γ Response Is Associated to Time Exposure Among Asymptomatic Immune Responders That Visited American Tegumentary Leishmaniasis Endemic Areas in Peru
Clinical manifestations of American Tegumentary Leishmaniasis (ATL) include cutaneous (CL) and mucous forms (ML); however, there are asymptomatic individuals who despite being infected do not present any clinical manifestations. This study characterized the cell-mediated immunity of travelers who li...
Guardado en:
| Autores principales: | , , , , , , |
|---|---|
| Formato: | Artículo |
| Lenguaje: | inglés |
| Publicado: |
2018
|
| Materias: | |
| Acceso en línea: | Ver en el sistema de origen Ver en el sistema de origen Enlace permanente (DOI o Handle) Enlace permanente (DOI o Handle) |
| Etiquetas: |
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| _version_ | 1881509003856445440 |
|---|---|
| author | Iván Best Angela Privat‐Maldonado María Cruz Mirko Zimic Rachel Bras‐Gonçalves Jean-Loup Lemesre Jorge Arévalo |
| author_browse | Angela Privat‐Maldonado Iván Best Jean-Loup Lemesre Jorge Arévalo María Cruz Mirko Zimic Rachel Bras‐Gonçalves |
| author_facet | Iván Best Angela Privat‐Maldonado María Cruz Mirko Zimic Rachel Bras‐Gonçalves Jean-Loup Lemesre Jorge Arévalo |
| author_sort | Iván Best |
| collection | Producción científica |
| description | Clinical manifestations of American Tegumentary Leishmaniasis (ATL) include cutaneous (CL) and mucous forms (ML); however, there are asymptomatic individuals who despite being infected do not present any clinical manifestations. This study characterized the cell-mediated immunity of travelers who lived in the Andean highlands of Cusco, free of leishmaniasis transmission, which eventually visited leishmaniasis endemic in the Amazonian basin and returned home without any clinical signs of the disease. Their immune response was compared with CL and ML patients who acquired the disease during their stage in the same region. Fifty-four human subjects from the highlands of Cusco (Peru), who have visited an endemic area, were enrolled: 28 of them did not show any symptoms, 12 showed CL and 14 showed ML. Ten healthy subjects from a non-endemic area (HS) were included as controls. T-cell proliferation was evaluated using peripheral blood mononuclear cells (PBMC) stimulated for 5 days with a total soluble leishmanial antigen (TSLA) of L. (V.) braziliensis. Th1/Th2/Th17 cytokines were also quantified in the supernatants by a flow cytometry multiplex assay. T-cell proliferation was expressed as stimulation index (SI) and the cut off was fixed at SI > 2.47. Fifteen out of 28 subjects did not show any signs of disease (54%); subjects with an SI above the cut off. They were defined as asymptomatic immune responders (AIR). CL and ML patients presented a higher SI than HS and AIR. Among the latter group, the exposure time to Leishmania was clearly associated with the IFN-γ response. Increased levels of this cytokine were observed in individuals who remained less than 90 days in an endemic area of leishmaniasis. Our results evidenced two sub-populations among asymptomatic individuals, one AIR who did not develop clinical disease manifestations when they were exposed to Leishmania in endemic areas. Exposure time to Leishmania in the wild was associated with the IFN-γ response. |
| format | Article |
| id | oai:cris.upeu.edu.pe:20.500.15037/927 |
| institution | UPeU |
| language | English |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| record_format | dspace |
| spelling | oai:cris.upeu.edu.pe:20.500.15037/9272026-10-30T16:14:59ZIFN-γ Response Is Associated to Time Exposure Among Asymptomatic Immune Responders That Visited American Tegumentary Leishmaniasis Endemic Areas in PeruIván BestAngela Privat‐MaldonadoMaría CruzMirko ZimicRachel Bras‐GonçalvesJean-Loup LemesreJorge ArévaloAsymptomaticImmune systemImmunologyLeishmaniasisMedicinePeripheral blood mononuclear cellLeishmaniaCellular immunityT cellCutaneous leishmaniasisImmunityInternal medicineBiologyParasite hostingComputer scienceBiochemistryIn vitroWorld Wide WebAsymptomaticImmune systemImmunologyLeishmaniasisMedicinePeripheral blood mononuclear cellLeishmaniaCellular immunityT cellCutaneous leishmaniasisImmunityInternal medicineBiologyParasite hostingEnvironmental ExposureEnvironmental ExposureEnvironmental ExposureHumansHumansHumansInterferon-gamma metabolismInterferon-gamma metabolismInterferon-gamma metabolismLeishmania immunologyLeishmania immunologyLeishmania immunologyPeruPeruPeruTime FactorsTime FactorsTime FactorsTravelTravelTravelT-Lymphocyte Subsets immunologyT-Lymphocyte Subsets immunologyT-Lymphocyte Subsets immunologyCytokines analysisCytokines analysisCytokines analysisLeishmaniasis, Cutaneous immunologyLeishmaniasis, Cutaneous immunologyLeishmaniasis, Cutaneous immunologyLeishmaniasis, Cutaneous pathologyLeishmaniasis, Cutaneous pathologyLeishmaniasis, Cutaneous pathologyCell ProliferationCell ProliferationCell ProliferationAsymptomatic DiseasesAsymptomatic DiseasesAsymptomatic DiseasesHealth Sciences Medicine Public Health, Environmental and Occupational Health Research on Leishmaniasis StudiesHealth Sciences Medicine Epidemiology Trypanosoma species research and implicationsClinical manifestations of American Tegumentary Leishmaniasis (ATL) include cutaneous (CL) and mucous forms (ML); however, there are asymptomatic individuals who despite being infected do not present any clinical manifestations. This study characterized the cell-mediated immunity of travelers who lived in the Andean highlands of Cusco, free of leishmaniasis transmission, which eventually visited leishmaniasis endemic in the Amazonian basin and returned home without any clinical signs of the disease. Their immune response was compared with CL and ML patients who acquired the disease during their stage in the same region. Fifty-four human subjects from the highlands of Cusco (Peru), who have visited an endemic area, were enrolled: 28 of them did not show any symptoms, 12 showed CL and 14 showed ML. Ten healthy subjects from a non-endemic area (HS) were included as controls. T-cell proliferation was evaluated using peripheral blood mononuclear cells (PBMC) stimulated for 5 days with a total soluble leishmanial antigen (TSLA) of L. (V.) braziliensis. Th1/Th2/Th17 cytokines were also quantified in the supernatants by a flow cytometry multiplex assay. T-cell proliferation was expressed as stimulation index (SI) and the cut off was fixed at SI > 2.47. Fifteen out of 28 subjects did not show any signs of disease (54%); subjects with an SI above the cut off. They were defined as asymptomatic immune responders (AIR). CL and ML patients presented a higher SI than HS and AIR. Among the latter group, the exposure time to Leishmania was clearly associated with the IFN-γ response. Increased levels of this cytokine were observed in individuals who remained less than 90 days in an endemic area of leishmaniasis. Our results evidenced two sub-populations among asymptomatic individuals, one AIR who did not develop clinical disease manifestations when they were exposed to Leishmania in endemic areas. Exposure time to Leishmania in the wild was associated with the IFN-γ response.Articlehttps://pubmed.ncbi.nlm.nih.gov/30186774https://www.frontiersin.org/articles/10.3389/fcimb.2018.00289/pdfhttps://doi.org/10.3389/fcimb.2018.0028910.3389/fcimb.2018.00289https://hdl.handle.net/20.500.15037/927W2888713590enFrontiers in Cellular and Infection Microbiology2235-29882018-08-212026-06-13T09:40:41Z2026-06-13T09:40:41Z |
| spellingShingle | Asymptomatic Immune system Immunology Leishmaniasis Medicine Peripheral blood mononuclear cell Leishmania Cellular immunity T cell Cutaneous leishmaniasis Immunity Internal medicine Biology Parasite hosting Computer science Biochemistry In vitro World Wide Web Asymptomatic Immune system Immunology Leishmaniasis Medicine Peripheral blood mononuclear cell Leishmania Cellular immunity T cell Cutaneous leishmaniasis Immunity Internal medicine Biology Parasite hosting Environmental Exposure Environmental Exposure Environmental Exposure Humans Humans Humans Interferon-gamma metabolism Interferon-gamma metabolism Interferon-gamma metabolism Leishmania immunology Leishmania immunology Leishmania immunology Peru Peru Peru Time Factors Time Factors Time Factors Travel Travel Travel T-Lymphocyte Subsets immunology T-Lymphocyte Subsets immunology T-Lymphocyte Subsets immunology Cytokines analysis Cytokines analysis Cytokines analysis Leishmaniasis, Cutaneous immunology Leishmaniasis, Cutaneous immunology Leishmaniasis, Cutaneous immunology Leishmaniasis, Cutaneous pathology Leishmaniasis, Cutaneous pathology Leishmaniasis, Cutaneous pathology Cell Proliferation Cell Proliferation Cell Proliferation Asymptomatic Diseases Asymptomatic Diseases Asymptomatic Diseases Health Sciences Medicine Public Health, Environmental and Occupational Health Research on Leishmaniasis Studies Health Sciences Medicine Epidemiology Trypanosoma species research and implications Iván Best Angela Privat‐Maldonado María Cruz Mirko Zimic Rachel Bras‐Gonçalves Jean-Loup Lemesre Jorge Arévalo IFN-γ Response Is Associated to Time Exposure Among Asymptomatic Immune Responders That Visited American Tegumentary Leishmaniasis Endemic Areas in Peru |
| title | IFN-γ Response Is Associated to Time Exposure Among Asymptomatic Immune Responders That Visited American Tegumentary Leishmaniasis Endemic Areas in Peru |
| title_full | IFN-γ Response Is Associated to Time Exposure Among Asymptomatic Immune Responders That Visited American Tegumentary Leishmaniasis Endemic Areas in Peru |
| title_fullStr | IFN-γ Response Is Associated to Time Exposure Among Asymptomatic Immune Responders That Visited American Tegumentary Leishmaniasis Endemic Areas in Peru |
| title_full_unstemmed | IFN-γ Response Is Associated to Time Exposure Among Asymptomatic Immune Responders That Visited American Tegumentary Leishmaniasis Endemic Areas in Peru |
| title_short | IFN-γ Response Is Associated to Time Exposure Among Asymptomatic Immune Responders That Visited American Tegumentary Leishmaniasis Endemic Areas in Peru |
| title_sort | ifn γ response is associated to time exposure among asymptomatic immune responders that visited american tegumentary leishmaniasis endemic areas in peru |
| topic | Asymptomatic Immune system Immunology Leishmaniasis Medicine Peripheral blood mononuclear cell Leishmania Cellular immunity T cell Cutaneous leishmaniasis Immunity Internal medicine Biology Parasite hosting Computer science Biochemistry In vitro World Wide Web Asymptomatic Immune system Immunology Leishmaniasis Medicine Peripheral blood mononuclear cell Leishmania Cellular immunity T cell Cutaneous leishmaniasis Immunity Internal medicine Biology Parasite hosting Environmental Exposure Environmental Exposure Environmental Exposure Humans Humans Humans Interferon-gamma metabolism Interferon-gamma metabolism Interferon-gamma metabolism Leishmania immunology Leishmania immunology Leishmania immunology Peru Peru Peru Time Factors Time Factors Time Factors Travel Travel Travel T-Lymphocyte Subsets immunology T-Lymphocyte Subsets immunology T-Lymphocyte Subsets immunology Cytokines analysis Cytokines analysis Cytokines analysis Leishmaniasis, Cutaneous immunology Leishmaniasis, Cutaneous immunology Leishmaniasis, Cutaneous immunology Leishmaniasis, Cutaneous pathology Leishmaniasis, Cutaneous pathology Leishmaniasis, Cutaneous pathology Cell Proliferation Cell Proliferation Cell Proliferation Asymptomatic Diseases Asymptomatic Diseases Asymptomatic Diseases Health Sciences Medicine Public Health, Environmental and Occupational Health Research on Leishmaniasis Studies Health Sciences Medicine Epidemiology Trypanosoma species research and implications |
| topic_facet | Asymptomatic Immune system Immunology Leishmaniasis Medicine Peripheral blood mononuclear cell Leishmania Cellular immunity T cell Cutaneous leishmaniasis Immunity Internal medicine Biology Parasite hosting Computer science Biochemistry In vitro World Wide Web Asymptomatic Immune system Immunology Leishmaniasis Medicine Peripheral blood mononuclear cell Leishmania Cellular immunity T cell Cutaneous leishmaniasis Immunity Internal medicine Biology Parasite hosting Environmental Exposure Environmental Exposure Environmental Exposure Humans Humans Humans Interferon-gamma metabolism Interferon-gamma metabolism Interferon-gamma metabolism Leishmania immunology Leishmania immunology Leishmania immunology Peru Peru Peru Time Factors Time Factors Time Factors Travel Travel Travel T-Lymphocyte Subsets immunology T-Lymphocyte Subsets immunology T-Lymphocyte Subsets immunology Cytokines analysis Cytokines analysis Cytokines analysis Leishmaniasis, Cutaneous immunology Leishmaniasis, Cutaneous immunology Leishmaniasis, Cutaneous immunology Leishmaniasis, Cutaneous pathology Leishmaniasis, Cutaneous pathology Leishmaniasis, Cutaneous pathology Cell Proliferation Cell Proliferation Cell Proliferation Asymptomatic Diseases Asymptomatic Diseases Asymptomatic Diseases Health Sciences Medicine Public Health, Environmental and Occupational Health Research on Leishmaniasis Studies Health Sciences Medicine Epidemiology Trypanosoma species research and implications |
| url | https://pubmed.ncbi.nlm.nih.gov/30186774 https://www.frontiersin.org/articles/10.3389/fcimb.2018.00289/pdf https://doi.org/10.3389/fcimb.2018.00289 https://hdl.handle.net/20.500.15037/927 |
| work_keys_str_mv | AT ivanbest ifngresponseisassociatedtotimeexposureamongasymptomaticimmunerespondersthatvisitedamericantegumentaryleishmaniasisendemicareasinperu AT angelaprivatmaldonado ifngresponseisassociatedtotimeexposureamongasymptomaticimmunerespondersthatvisitedamericantegumentaryleishmaniasisendemicareasinperu AT mariacruz ifngresponseisassociatedtotimeexposureamongasymptomaticimmunerespondersthatvisitedamericantegumentaryleishmaniasisendemicareasinperu AT mirkozimic ifngresponseisassociatedtotimeexposureamongasymptomaticimmunerespondersthatvisitedamericantegumentaryleishmaniasisendemicareasinperu AT rachelbrasgoncalves ifngresponseisassociatedtotimeexposureamongasymptomaticimmunerespondersthatvisitedamericantegumentaryleishmaniasisendemicareasinperu AT jeanlouplemesre ifngresponseisassociatedtotimeexposureamongasymptomaticimmunerespondersthatvisitedamericantegumentaryleishmaniasisendemicareasinperu AT jorgearevalo ifngresponseisassociatedtotimeexposureamongasymptomaticimmunerespondersthatvisitedamericantegumentaryleishmaniasisendemicareasinperu |