Tracking of quiescence in Leishmania by quantifying the expression of GFP in the ribosomal DNA locus

Under stressful conditions some microorganisms adopt a quiescent stage characterized by a reversible non or slow proliferative condition that allows their survival. This adaptation was only recently discovered in Leishmania. We developed an in vitro model and a biosensor to track quiescence at popul...

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Autores principales: Marlene Jara, Ilse Maes, Hideo Imamura, Malgorzata A. Domagalska, Jean‐Claude Dujardin, Jorge Arévalo
Formato: Artículo
Lenguaje:inglés
Publicado: 2019
Materias:
Amastigote
Biology
Green fluorescent protein
Population
Cell biology
Gene expression
Locus (genetics)
Leishmania
Gene
Reporter gene
Molecular biology
Genetics
Parasite hosting
World Wide Web
Computer science
Sociology
Demography
Microorganisms, Genetically-Modified
Animals
DNA, Ribosomal genetics
DNA, Ribosomal metabolism
Leishmania braziliensis cytology
Leishmania braziliensis growth & development
Leishmania braziliensis metabolism
Leishmania mexicana cytology
Leishmania mexicana genetics
Leishmania mexicana metabolism
DNA, Protozoan genetics
DNA, Protozoan metabolism
Green Fluorescent Proteins biosynthesis
Green Fluorescent Proteins genetics
Mice
Genetic Loci
Health Sciences Medicine Public Health, Environmental and Occupational Health Research on Leishmaniasis Studies
Health Sciences Medicine Epidemiology Trypanosoma species research and implications
Life Sciences Biochemistry, Genetics and Molecular Biology Molecular Biology Biochemical and Molecular Research
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Internet

https://pubmed.ncbi.nlm.nih.gov/31831818
https://www.nature.com/articles/s41598-019-55486-z.pdf
https://doi.org/10.1038/s41598-019-55486-z
https://hdl.handle.net/20.500.15037/923

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