LAMP-coupled CRISPR-Cas12a assays: A promising new tool for molecular diagnosis of leishmaniasis

BACKGROUND: Tegumentary leishmaniasis is a parasitic disease endemic in the Americas. Its clinical management and control rely on early and accurate diagnosis and adequate treatment. PCR-based molecular diagnostics offer high sensitivity and specificity over microscopy or culture but are less access...

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Guardado en:
Autores principales: Eva Dueñas, Ingrid Tirado, Percy Huaihua, Ariana Parra del Riego, Luis Cabrera-Sosa, Jose A. Nakamoto, Maria Manuel Cruz, Carlos M. Restrepo, Jorge Arévalo, Vanessa Adaui
Formato: Artículo
Lenguaje:inglés
Publicado: 2026
Materias:
Leishmaniasis
Leishmania
Disease
Medicine
Tropical disease
Cutaneous leishmaniasis
Visceral leishmaniasis
Neglected tropical diseases
Computational biology
Molecular diagnostics
Immunology
Virology
Protozoal disease
Biology
Zoonotic disease
Diagnostic test
Humans
Leishmania genetics
Leishmania isolation & purification
Leishmania braziliensis genetics
Leishmania braziliensis isolation & purification
Leishmaniasis diagnosis
Leishmaniasis parasitology
RNA, Ribosomal, 18S genetics
Sensitivity and Specificity
DNA, Protozoan genetics
Leishmaniasis, Cutaneous diagnosis
Leishmaniasis, Cutaneous parasitology
DNA, Kinetoplast genetics
Nucleic Acid Amplification Techniques methods
Molecular Diagnostic Techniques methods
CRISPR-Cas Systems
Health Sciences Medicine Public Health, Environmental and Occupational Health Research on Leishmaniasis Studies
Health Sciences Medicine Epidemiology Trypanosoma species research and implications
Life Sciences Immunology and Microbiology Parasitology Parasites and Host Interactions
Acceso en línea:Ver en el sistema de origen
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Internet

https://pubmed.ncbi.nlm.nih.gov/41686849
https://doi.org/10.1371/journal.pntd.0013456
https://hdl.handle.net/20.500.15037/886

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