A Novel Fast and Efficient Approach to Purify the Thrombin-like Enzyme from Two Bothrops-genus Snake Venoms
Snake venoms are important sources of complex substances with a variety of pharmacological activities. Among them serine proteinases (SVSPs) have important effects on the hemostatic system influencing the hemodynamic of human or animal blood. Bothrops genus-snake venoms are rich in the thrombin-like...
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2021
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| author | Aurelio Gomes Heleno, Mauricio Newball Noriega, Edda Evnet Huancahuire Vega, Salomon Seabra Ferreira Júnior, Rui Barraviera, Benedito |
| author_browse | Aurelio Gomes Heleno, Mauricio Barraviera, Benedito Huancahuire Vega, Salomon Newball Noriega, Edda Evnet Seabra Ferreira Júnior, Rui |
| author_facet | Aurelio Gomes Heleno, Mauricio Newball Noriega, Edda Evnet Huancahuire Vega, Salomon Seabra Ferreira Júnior, Rui Barraviera, Benedito |
| author_sort | Aurelio Gomes Heleno, Mauricio |
| collection | Producción científica |
| description | Snake venoms are important sources of complex substances with a variety of pharmacological activities. Among them serine proteinases (SVSPs) have important effects on the hemostatic system influencing the hemodynamic of human or animal blood. Bothrops genus-snake venoms are rich in the thrombin-like enzyme, a type of SVSPs, with great interest to produce medicine. Therefore, the aim of this work was to describe a rapid, only two-step chromatographic-procedure developed to perform a faster purification of SVSPs from Bothrops alternatus and Bothrops moojeni venoms. As a result, two groups of serine proteinases respectively BaIII-4 - 8 and BmIII-2 - 5, were isolated and their molecular masses estimated by mass spectrometry and SDS-PAGE under denaturing conditions. The SVTLEs isolated from B. alternatus (BaIII-3 - 8) and B. moojeni (BmIII-2 - 5) fractions displayed apparent molecular mass around 30-40 kDa which closely relates to SVTLEs from other Bothrops species, as well their amino acid partial sequence triptych ions. Analysis of the alignment of the amino acid residue sequences of the N-terminal of the isolated proteins revealed a high level of identity with other SVTLEs. These enzymes coagulated plasma and showed fibrinogenolytic activity in blood. These SVTLEs isolated can be considered α-fibrinogenase mainly due to the fact that they hydrolyze the Aα chain fibrinogen. B. moojeni SVTLE showed greater activity than those from B. alternatus isolated. This new purification alternative approach developed was faster and more economical than the traditional process currently used. Faster purification and improved extraction yield can provide new insights into these enzymes including the use as a candidate molecule in the production of new drugs. |
| format | Article |
| id | oai:cris.upeu.edu.pe:20.500.15037/488 |
| institution | UPeU |
| language | English |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| record_format | dspace |
| spelling | oai:cris.upeu.edu.pe:20.500.15037/4882026-11-02T12:57:04ZA Novel Fast and Efficient Approach to Purify the Thrombin-like Enzyme from Two Bothrops-genus Snake VenomsAurelio Gomes Heleno, MauricioNewball Noriega, Edda EvnetHuancahuire Vega, SalomonSeabra Ferreira Júnior, RuiBarraviera, BeneditoBothrops alternatusBothrops moojeniSerine ProteinasesThrombin-like EnzymeFast PurificationSnake venoms are important sources of complex substances with a variety of pharmacological activities. Among them serine proteinases (SVSPs) have important effects on the hemostatic system influencing the hemodynamic of human or animal blood. Bothrops genus-snake venoms are rich in the thrombin-like enzyme, a type of SVSPs, with great interest to produce medicine. Therefore, the aim of this work was to describe a rapid, only two-step chromatographic-procedure developed to perform a faster purification of SVSPs from Bothrops alternatus and Bothrops moojeni venoms. As a result, two groups of serine proteinases respectively BaIII-4 - 8 and BmIII-2 - 5, were isolated and their molecular masses estimated by mass spectrometry and SDS-PAGE under denaturing conditions. The SVTLEs isolated from B. alternatus (BaIII-3 - 8) and B. moojeni (BmIII-2 - 5) fractions displayed apparent molecular mass around 30-40 kDa which closely relates to SVTLEs from other Bothrops species, as well their amino acid partial sequence triptych ions. Analysis of the alignment of the amino acid residue sequences of the N-terminal of the isolated proteins revealed a high level of identity with other SVTLEs. These enzymes coagulated plasma and showed fibrinogenolytic activity in blood. These SVTLEs isolated can be considered α-fibrinogenase mainly due to the fact that they hydrolyze the Aα chain fibrinogen. B. moojeni SVTLE showed greater activity than those from B. alternatus isolated. This new purification alternative approach developed was faster and more economical than the traditional process currently used. Faster purification and improved extraction yield can provide new insights into these enzymes including the use as a candidate molecule in the production of new drugs.Articlehttp://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbls.20210904.15.pdfhttps://doi.org/10.11648/j.ajbls.20210904.1510.11648/j.ajbls.20210904.15https://hdl.handle.net/20.500.15037/488W3199785448enAmerican Journal of Biomedical and Life Sciences2330-880X2330-88182021-01-012026-06-13T08:14:51Z2026-06-13T08:14:51Z |
| spellingShingle | Bothrops alternatus Bothrops moojeni Serine Proteinases Thrombin-like Enzyme Fast Purification Aurelio Gomes Heleno, Mauricio Newball Noriega, Edda Evnet Huancahuire Vega, Salomon Seabra Ferreira Júnior, Rui Barraviera, Benedito A Novel Fast and Efficient Approach to Purify the Thrombin-like Enzyme from Two Bothrops-genus Snake Venoms |
| title | A Novel Fast and Efficient Approach to Purify the Thrombin-like Enzyme from Two Bothrops-genus Snake Venoms |
| title_full | A Novel Fast and Efficient Approach to Purify the Thrombin-like Enzyme from Two Bothrops-genus Snake Venoms |
| title_fullStr | A Novel Fast and Efficient Approach to Purify the Thrombin-like Enzyme from Two Bothrops-genus Snake Venoms |
| title_full_unstemmed | A Novel Fast and Efficient Approach to Purify the Thrombin-like Enzyme from Two Bothrops-genus Snake Venoms |
| title_short | A Novel Fast and Efficient Approach to Purify the Thrombin-like Enzyme from Two Bothrops-genus Snake Venoms |
| title_sort | novel fast and efficient approach to purify the thrombin like enzyme from two bothrops genus snake venoms |
| topic | Bothrops alternatus Bothrops moojeni Serine Proteinases Thrombin-like Enzyme Fast Purification |
| topic_facet | Bothrops alternatus Bothrops moojeni Serine Proteinases Thrombin-like Enzyme Fast Purification |
| url | http://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbls.20210904.15.pdf https://doi.org/10.11648/j.ajbls.20210904.15 https://hdl.handle.net/20.500.15037/488 |
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