Tolerance to disinfectants (chlorhexidine and isopropanol) and its association with antibiotic resistance in clinically-related Klebsiella pneumoniae isolates
Disinfectants play an essential role in controlling the dissemination of bacteria in health care settings, but it may also contribute to the selection of antibiotic resistance bacteria. This study looked at Klebsiella pneumoniae isolates collected from three hospitals in Lima, Peru, in order to eval...
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Taylor and Francis Ltd.
2021
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| author | Morante, Jasmine Quispe, Antonio M. Ymaña, Barbara Moya, Jeel Junior Luque Chipana, Nestor Alejandro Soza, Gabriela Ramos-Chirinos, María Pons, María Jesus |
| author_browse | Luque Chipana, Nestor Alejandro Morante, Jasmine Moya, Jeel Junior Pons, María Jesus Quispe, Antonio M. Ramos-Chirinos, María Soza, Gabriela Ymaña, Barbara |
| author_facet | Morante, Jasmine Quispe, Antonio M. Ymaña, Barbara Moya, Jeel Junior Luque Chipana, Nestor Alejandro Soza, Gabriela Ramos-Chirinos, María Pons, María Jesus |
| author_sort | Morante, Jasmine |
| collection | Producción científica |
| description | Disinfectants play an essential role in controlling the dissemination of bacteria in health care settings, but it may also contribute to the selection of antibiotic resistance bacteria. This study looked at Klebsiella pneumoniae isolates collected from three hospitals in Lima, Peru, in order to evaluate: their susceptibility to chlorhexidine [CHG] and isopropanol [ISP]), and their association with antimicrobial susceptibility. We analyzed 59 K. pneumoniae isolates and assessed their CHG and ISP susceptibility by minimum inhibitory concentrations (MICs). Additionally, we performed a regression analysis to assess the association between disinfectant tolerance and antibiotic resistance (measured by the disc diffusion method), colistin resistance (by microdilution), carbapenemases presence (by polymerase chain reaction [PCR]), and clonal relationships (by pulsed-field gel electrophoresis [PFGE]). Eleven K. pneumoniae strains were isolated from fomites, and 48 strains from clinical samples. The MIC range of these isolates was 8-128 µg/ml for CHG and 16-256 mg/ml for ISP. We found that resistance to trimethoprim/sulfamethoxazole (TMP/SMX) was the main factor associated with CHG log2 MIC (ß = 0.65; 95%CI: 0.03, 1.27; R2 = 0.07). In the case of ISP, the log2(MIC) was associated with the institution of origin, showing lower ISP log2(MIC) in fomites compared to clinical samples(ß = -0.77; 95%CI: -1.54, -0.01; R2 = 0.08). Resistance to CHG and ISP among K. pneumoniae isolates found in Peruvian hospitals seems to be elevated and highly variable. Further studies are needed to confirm our results and implement actionable interventions if necessary. |
| format | Article |
| id | oai:cris.upeu.edu.pe:20.500.15037/3205 |
| institution | UPeU |
| language | English |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | Taylor and Francis Ltd. |
| publisherStr | Taylor and Francis Ltd. |
| record_format | dspace |
| spelling | oai:cris.upeu.edu.pe:20.500.15037/32052026-10-30T16:20:18ZTolerance to disinfectants (chlorhexidine and isopropanol) and its association with antibiotic resistance in clinically-related Klebsiella pneumoniae isolatesMorante, JasmineQuispe, Antonio M.Ymaña, BarbaraMoya, Jeel JuniorLuque Chipana, Nestor AlejandroSoza, GabrielaRamos-Chirinos, MaríaPons, María JesusCross-resistanceDisinfectantsAntibiotic ResistanceChlorhexidineKkebsiella PneumoniaeAlcohol-based DisinfectantsDisinfectants play an essential role in controlling the dissemination of bacteria in health care settings, but it may also contribute to the selection of antibiotic resistance bacteria. This study looked at Klebsiella pneumoniae isolates collected from three hospitals in Lima, Peru, in order to evaluate: their susceptibility to chlorhexidine [CHG] and isopropanol [ISP]), and their association with antimicrobial susceptibility. We analyzed 59 K. pneumoniae isolates and assessed their CHG and ISP susceptibility by minimum inhibitory concentrations (MICs). Additionally, we performed a regression analysis to assess the association between disinfectant tolerance and antibiotic resistance (measured by the disc diffusion method), colistin resistance (by microdilution), carbapenemases presence (by polymerase chain reaction [PCR]), and clonal relationships (by pulsed-field gel electrophoresis [PFGE]). Eleven K. pneumoniae strains were isolated from fomites, and 48 strains from clinical samples. The MIC range of these isolates was 8-128 µg/ml for CHG and 16-256 mg/ml for ISP. We found that resistance to trimethoprim/sulfamethoxazole (TMP/SMX) was the main factor associated with CHG log2 MIC (ß = 0.65; 95%CI: 0.03, 1.27; R2 = 0.07). In the case of ISP, the log2(MIC) was associated with the institution of origin, showing lower ISP log2(MIC) in fomites compared to clinical samples(ß = -0.77; 95%CI: -1.54, -0.01; R2 = 0.08). Resistance to CHG and ISP among K. pneumoniae isolates found in Peruvian hospitals seems to be elevated and highly variable. Further studies are needed to confirm our results and implement actionable interventions if necessary.Article10.1080/20477724.2020.18454792-s2.0-85099927921https://hdl.handle.net/20.500.15037/3205enPathogens and Global HealthPathogens and Global Health2047-7724Taylor and Francis Ltd.2021-01-022026-06-27T02:36:28Z2026-06-27T02:36:28Z |
| spellingShingle | Cross-resistance Disinfectants Antibiotic Resistance Chlorhexidine Kkebsiella Pneumoniae Alcohol-based Disinfectants Morante, Jasmine Quispe, Antonio M. Ymaña, Barbara Moya, Jeel Junior Luque Chipana, Nestor Alejandro Soza, Gabriela Ramos-Chirinos, María Pons, María Jesus Tolerance to disinfectants (chlorhexidine and isopropanol) and its association with antibiotic resistance in clinically-related Klebsiella pneumoniae isolates |
| title | Tolerance to disinfectants (chlorhexidine and isopropanol) and its association with antibiotic resistance in clinically-related Klebsiella pneumoniae isolates |
| title_full | Tolerance to disinfectants (chlorhexidine and isopropanol) and its association with antibiotic resistance in clinically-related Klebsiella pneumoniae isolates |
| title_fullStr | Tolerance to disinfectants (chlorhexidine and isopropanol) and its association with antibiotic resistance in clinically-related Klebsiella pneumoniae isolates |
| title_full_unstemmed | Tolerance to disinfectants (chlorhexidine and isopropanol) and its association with antibiotic resistance in clinically-related Klebsiella pneumoniae isolates |
| title_short | Tolerance to disinfectants (chlorhexidine and isopropanol) and its association with antibiotic resistance in clinically-related Klebsiella pneumoniae isolates |
| title_sort | tolerance to disinfectants chlorhexidine and isopropanol and its association with antibiotic resistance in clinically related klebsiella pneumoniae isolates |
| topic | Cross-resistance Disinfectants Antibiotic Resistance Chlorhexidine Kkebsiella Pneumoniae Alcohol-based Disinfectants |
| topic_facet | Cross-resistance Disinfectants Antibiotic Resistance Chlorhexidine Kkebsiella Pneumoniae Alcohol-based Disinfectants |
| url | https://hdl.handle.net/20.500.15037/3205 |
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