Sperm chromatin stability and their relationship with fertilization rate in Sheep of the Junín race
The objective of this research was to evaluate the effect of sperm on chromatin stability and its relationship with the membrane integrity structural – physiological and the rate of fertilization of female sheep. Ejaculates of sperm (2 × 109 sperm·mL-1) with 70% of motility were collected using an a...
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Universidad del Zulia, Facultad de Ciencias Veterinarias
2022
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| author | Unchupaico-Payano, Ide Alponte-Sierra, Alberto Quispe, Carlos Ancco, Edith Huaman de la Cruz, Alex Ruben Mariños-Alfaro, Julio César Patiño-Rivera, Alberto Rivelino Lavado-Meza, Carmencita R. Huanca-Rojas, Lupe Bazán-Alonso, Luis |
| author_browse | Alponte-Sierra, Alberto Ancco, Edith Bazán-Alonso, Luis Huaman de la Cruz, Alex Ruben Huanca-Rojas, Lupe Lavado-Meza, Carmencita R. Mariños-Alfaro, Julio César Patiño-Rivera, Alberto Rivelino Quispe, Carlos Unchupaico-Payano, Ide |
| author_facet | Unchupaico-Payano, Ide Alponte-Sierra, Alberto Quispe, Carlos Ancco, Edith Huaman de la Cruz, Alex Ruben Mariños-Alfaro, Julio César Patiño-Rivera, Alberto Rivelino Lavado-Meza, Carmencita R. Huanca-Rojas, Lupe Bazán-Alonso, Luis |
| author_sort | Unchupaico-Payano, Ide |
| collection | Producción científica |
| description | The objective of this research was to evaluate the effect of sperm on chromatin stability and its relationship with the membrane integrity structural – physiological and the rate of fertilization of female sheep. Ejaculates of sperm (2 × 109 sperm·mL-1) with 70% of motility were collected using an artificial vagina (n=5, 2 years old. For this, each ram was served with fifteen female sheep (n=75), generating thus five different Groups (A, B, C, D, and E). A control Group also was considered. Sperm nuclear chromatin stability (NCS) was evaluated using the Borate Buffer (BB), Sodium Dodecyl Sulfate (SDS), and the mixture of Ethylenediaminetetraacetic acid (EDTA) + SDS. The fertilization rate was evaluated after 16-18 hours post sperm injection. Sperm concentration showed a significant difference (P<0.05) between Groups. In Contrast, seminal volume, and sperm motility do not show a significant difference (P>0.05). A high correlation (r2=0.52) was observed between morphology and motility, and the fertilization rate was 74.6% (n=56). It was concluded in general that techniques to evaluate nuclear condensation values do have a high likelihood to give a diagnosis about the future potential of sperm populations in Junín ram. |
| format | Article |
| id | oai:cris.upeu.edu.pe:20.500.15037/3131 |
| institution | UPeU |
| language | Spanish |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | Universidad del Zulia, Facultad de Ciencias Veterinarias |
| publisherStr | Universidad del Zulia, Facultad de Ciencias Veterinarias |
| record_format | dspace |
| spelling | oai:cris.upeu.edu.pe:20.500.15037/31312026-10-30T17:10:06ZSperm chromatin stability and their relationship with fertilization rate in Sheep of the Junín raceEstabilidad de la cromatina espermática y su relación con la tasa de fecundación en ovejas de la raza JunínUnchupaico-Payano, IdeAlponte-Sierra, AlbertoQuispe, CarlosAncco, EdithHuaman de la Cruz, Alex RubenMariños-Alfaro, Julio CésarPatiño-Rivera, Alberto RivelinoLavado-Meza, Carmencita R.Huanca-Rojas, LupeBazán-Alonso, LuisSperm chromatinmembrane integrityPeruvian ramsThe objective of this research was to evaluate the effect of sperm on chromatin stability and its relationship with the membrane integrity structural – physiological and the rate of fertilization of female sheep. Ejaculates of sperm (2 × 109 sperm·mL-1) with 70% of motility were collected using an artificial vagina (n=5, 2 years old. For this, each ram was served with fifteen female sheep (n=75), generating thus five different Groups (A, B, C, D, and E). A control Group also was considered. Sperm nuclear chromatin stability (NCS) was evaluated using the Borate Buffer (BB), Sodium Dodecyl Sulfate (SDS), and the mixture of Ethylenediaminetetraacetic acid (EDTA) + SDS. The fertilization rate was evaluated after 16-18 hours post sperm injection. Sperm concentration showed a significant difference (P<0.05) between Groups. In Contrast, seminal volume, and sperm motility do not show a significant difference (P>0.05). A high correlation (r2=0.52) was observed between morphology and motility, and the fertilization rate was 74.6% (n=56). It was concluded in general that techniques to evaluate nuclear condensation values do have a high likelihood to give a diagnosis about the future potential of sperm populations in Junín ram.Article10.52973/rcfcv-e321572-s2.0-85172872888https://hdl.handle.net/20.500.15037/3131esRevista Científica de la Facultad de Ciencias VeterinariasRevista Cientifica de la Facultad de Veterinaria0798-2259Universidad del Zulia, Facultad de Ciencias Veterinarias2022-09-282026-06-27T02:36:02Z2026-06-27T02:36:02Z |
| spellingShingle | Sperm chromatin membrane integrity Peruvian rams Unchupaico-Payano, Ide Alponte-Sierra, Alberto Quispe, Carlos Ancco, Edith Huaman de la Cruz, Alex Ruben Mariños-Alfaro, Julio César Patiño-Rivera, Alberto Rivelino Lavado-Meza, Carmencita R. Huanca-Rojas, Lupe Bazán-Alonso, Luis Sperm chromatin stability and their relationship with fertilization rate in Sheep of the Junín race |
| title | Sperm chromatin stability and their relationship with fertilization rate in Sheep of the Junín race |
| title_full | Sperm chromatin stability and their relationship with fertilization rate in Sheep of the Junín race |
| title_fullStr | Sperm chromatin stability and their relationship with fertilization rate in Sheep of the Junín race |
| title_full_unstemmed | Sperm chromatin stability and their relationship with fertilization rate in Sheep of the Junín race |
| title_short | Sperm chromatin stability and their relationship with fertilization rate in Sheep of the Junín race |
| title_sort | sperm chromatin stability and their relationship with fertilization rate in sheep of the junin race |
| topic | Sperm chromatin membrane integrity Peruvian rams |
| topic_facet | Sperm chromatin membrane integrity Peruvian rams |
| url | https://hdl.handle.net/20.500.15037/3131 |
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