Inhibitory Activity of Essential Oils of Mentha spicata and Eucalyptus globulus on Biofilms of Streptococcus mutans in an In Vitro Model
The aim of this study was to evaluate the inhibitory activity of the commercially available essential oils of Mentha spicata (spearmint) and Eucalyptus globulus (eucalyptus) on Streptococcus mutans ATCC 25175 biofilms in vitro, emulating dental plaque conditions. The composition of the essential oil...
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MDPI
2023
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| author | Landeo-Villanueva, Guillermo Ernesto Salazar-Salvatierra, María Elena Ruiz-Quiroz, Julio Reynaldo Zuta-Arriola, Noemi Jarama Soto, Benjamin Herrera-Calderon, Oscar Pari-Olarte, Josefa Bertha Loyola-Gonzales, Eddie |
| author_browse | Herrera-Calderon, Oscar Jarama Soto, Benjamin Landeo-Villanueva, Guillermo Ernesto Loyola-Gonzales, Eddie Pari-Olarte, Josefa Bertha Ruiz-Quiroz, Julio Reynaldo Salazar-Salvatierra, María Elena Zuta-Arriola, Noemi |
| author_facet | Landeo-Villanueva, Guillermo Ernesto Salazar-Salvatierra, María Elena Ruiz-Quiroz, Julio Reynaldo Zuta-Arriola, Noemi Jarama Soto, Benjamin Herrera-Calderon, Oscar Pari-Olarte, Josefa Bertha Loyola-Gonzales, Eddie |
| author_sort | Landeo-Villanueva, Guillermo Ernesto |
| collection | Producción científica |
| description | The aim of this study was to evaluate the inhibitory activity of the commercially available essential oils of Mentha spicata (spearmint) and Eucalyptus globulus (eucalyptus) on Streptococcus mutans ATCC 25175 biofilms in vitro, emulating dental plaque conditions. The composition of the essential oils (EOs) was determined using gas chromatography coupled with mass spectrometry (GC-MS), with the main metabolites being Carvone (57.93%) and Limonene (12.91%) for Mentha spicata and 1,8-Cineole (Eucalyptol) (65.83%) for Eucalyptus globulus. The inhibitory activity was evaluated using the methods of agar-well diffusion and colorimetric microdilution. The inhibition halos were 18.3 ± 0.47 mm and 27.0 ± 0.82 mm, and the MICs were 1.8484 mg/mL and 1.9168 mg/mL for the EOs of Mentha spicata and Eucalyptus globulus, respectively. The activity against the biofilms was evaluated on a substrate of bovine enamel pieces using a basal mucin medium (BMM) in anaerobic conditions with daily sucrose exposition cycles in order to emulate oral cavity conditions. The EOs were applied in a concentration of 0.5% in a sterile saline vehicle with 1% polysorbate 20. After 72 h of cultivation, a significant reduction was observed (p < 0.001%) on the biofilm biomass, which was evaluated by its turbidity in suspension and using a count of the recoverable organisms with regards to the control. The effects of the Eos were not significantly distinct from each other. The EOs showed antimicrobial activity against both the Streptococcus mutans planktonic and biofilm cultures. Thus, EOs may have great potential for the development of pharmaceutical and sanitary products for oral health. |
| format | Article |
| id | oai:cris.upeu.edu.pe:20.500.15037/3010 |
| institution | UPeU |
| language | English |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | MDPI |
| publisherStr | MDPI |
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| spelling | oai:cris.upeu.edu.pe:20.500.15037/30102026-10-30T17:09:37ZInhibitory Activity of Essential Oils of Mentha spicata and Eucalyptus globulus on Biofilms of Streptococcus mutans in an In Vitro ModelLandeo-Villanueva, Guillermo ErnestoSalazar-Salvatierra, María ElenaRuiz-Quiroz, Julio ReynaldoZuta-Arriola, NoemiJarama Soto, BenjaminHerrera-Calderon, OscarPari-Olarte, Josefa BerthaLoyola-Gonzales, EddieEssential oilsMedicinal plantsVolatile oilVolatile oilsbiofilmsDentistryAntibiofilmThe aim of this study was to evaluate the inhibitory activity of the commercially available essential oils of Mentha spicata (spearmint) and Eucalyptus globulus (eucalyptus) on Streptococcus mutans ATCC 25175 biofilms in vitro, emulating dental plaque conditions. The composition of the essential oils (EOs) was determined using gas chromatography coupled with mass spectrometry (GC-MS), with the main metabolites being Carvone (57.93%) and Limonene (12.91%) for Mentha spicata and 1,8-Cineole (Eucalyptol) (65.83%) for Eucalyptus globulus. The inhibitory activity was evaluated using the methods of agar-well diffusion and colorimetric microdilution. The inhibition halos were 18.3 ± 0.47 mm and 27.0 ± 0.82 mm, and the MICs were 1.8484 mg/mL and 1.9168 mg/mL for the EOs of Mentha spicata and Eucalyptus globulus, respectively. The activity against the biofilms was evaluated on a substrate of bovine enamel pieces using a basal mucin medium (BMM) in anaerobic conditions with daily sucrose exposition cycles in order to emulate oral cavity conditions. The EOs were applied in a concentration of 0.5% in a sterile saline vehicle with 1% polysorbate 20. After 72 h of cultivation, a significant reduction was observed (p < 0.001%) on the biofilm biomass, which was evaluated by its turbidity in suspension and using a count of the recoverable organisms with regards to the control. The effects of the Eos were not significantly distinct from each other. The EOs showed antimicrobial activity against both the Streptococcus mutans planktonic and biofilm cultures. Thus, EOs may have great potential for the development of pharmaceutical and sanitary products for oral health.Article10.3390/antibiotics120203692-s2.0-85148851611https://hdl.handle.net/20.500.15037/3010enAntibioticsAntibiotics2079-6382MDPI2023-02-102026-06-27T02:35:18Z2026-06-27T02:35:18Z |
| spellingShingle | Essential oils Medicinal plants Volatile oil Volatile oils biofilms Dentistry Antibiofilm Landeo-Villanueva, Guillermo Ernesto Salazar-Salvatierra, María Elena Ruiz-Quiroz, Julio Reynaldo Zuta-Arriola, Noemi Jarama Soto, Benjamin Herrera-Calderon, Oscar Pari-Olarte, Josefa Bertha Loyola-Gonzales, Eddie Inhibitory Activity of Essential Oils of Mentha spicata and Eucalyptus globulus on Biofilms of Streptococcus mutans in an In Vitro Model |
| title | Inhibitory Activity of Essential Oils of Mentha spicata and Eucalyptus globulus on Biofilms of Streptococcus mutans in an In Vitro Model |
| title_full | Inhibitory Activity of Essential Oils of Mentha spicata and Eucalyptus globulus on Biofilms of Streptococcus mutans in an In Vitro Model |
| title_fullStr | Inhibitory Activity of Essential Oils of Mentha spicata and Eucalyptus globulus on Biofilms of Streptococcus mutans in an In Vitro Model |
| title_full_unstemmed | Inhibitory Activity of Essential Oils of Mentha spicata and Eucalyptus globulus on Biofilms of Streptococcus mutans in an In Vitro Model |
| title_short | Inhibitory Activity of Essential Oils of Mentha spicata and Eucalyptus globulus on Biofilms of Streptococcus mutans in an In Vitro Model |
| title_sort | inhibitory activity of essential oils of mentha spicata and eucalyptus globulus on biofilms of streptococcus mutans in an in vitro model |
| topic | Essential oils Medicinal plants Volatile oil Volatile oils biofilms Dentistry Antibiofilm |
| topic_facet | Essential oils Medicinal plants Volatile oil Volatile oils biofilms Dentistry Antibiofilm |
| url | https://hdl.handle.net/20.500.15037/3010 |
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