Development of a Hybrid System Based on the CIELAB Colour Space and Artificial Neural Networks for Monitoring pH and Acidity During Yogurt Fermentation

Monitoring pH and acidity during yoghurt fermentation is essential for product quality and process efficiency. Conventional measurement methods, however, are invasive and labour-intensive. This study developed artificial neural network (ANN) models to predict pH and titratable acidity during yoghurt...

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Autores principales: Alvarado, Ulises, Tacuri, Jhon, Coloma, Alejandro, Gallegos Rojas, Edgar, Callo, Herbert, Valencia-Sullca, Cristina, Curasi Rafael, Nancy, Castillo, Manuel Z.
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Publicado: Multidisciplinary Digital Publishing Institute (MDPI) 2025
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author Alvarado, Ulises
Tacuri, Jhon
Coloma, Alejandro
Gallegos Rojas, Edgar
Callo, Herbert
Valencia-Sullca, Cristina
Curasi Rafael, Nancy
Castillo, Manuel Z.
author_browse Alvarado, Ulises
Callo, Herbert
Castillo, Manuel Z.
Coloma, Alejandro
Curasi Rafael, Nancy
Gallegos Rojas, Edgar
Tacuri, Jhon
Valencia-Sullca, Cristina
author_facet Alvarado, Ulises
Tacuri, Jhon
Coloma, Alejandro
Gallegos Rojas, Edgar
Callo, Herbert
Valencia-Sullca, Cristina
Curasi Rafael, Nancy
Castillo, Manuel Z.
author_sort Alvarado, Ulises
collection Producción científica
description Monitoring pH and acidity during yoghurt fermentation is essential for product quality and process efficiency. Conventional measurement methods, however, are invasive and labour-intensive. This study developed artificial neural network (ANN) models to predict pH and titratable acidity during yoghurt fermentation using CIELAB colour parameters (L, a*, b*). Reconstituted milk powder with 12% total solids was prepared with varying protein levels (4.2–4.8%), inoculum concentrations (1–3%), and fermentation temperatures (36–44 °C). Data were collected every 10 min until pH 4.6 was reached. Forty models were trained for each output variable, using 90% of the data for training and 10% for validation. The first two phases of the fermentation process were clearly distinguishable, lasting between 4.5 and 7 h and exceeding 0.6% lactic acid in all treatments evaluated. The best pH model used two hidden layers with 28 neurons (R2 = 0.969; RMSE = 0.007), while the optimal acidity model had four hidden layers with 32 neurons (R2 = 0.868; RMSE = 0.002). The strong correlation between colour and physicochemical changes confirms the feasibility of this non-destructive approach. Integrating ANN models and colourimetry offers a practical solution for real-time monitoring, helping improve process control in industrial yoghurt production.
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spelling oai:cris.upeu.edu.pe:20.500.15037/23712026-10-30T17:09:11ZDevelopment of a Hybrid System Based on the CIELAB Colour Space and Artificial Neural Networks for Monitoring pH and Acidity During Yogurt FermentationAlvarado, UlisesTacuri, JhonColoma, AlejandroGallegos Rojas, EdgarCallo, HerbertValencia-Sullca, CristinaCurasi Rafael, NancyCastillo, Manuel Z.fermentationpHacidityCIELAB colour spaceartificial neural networksMonitoring pH and acidity during yoghurt fermentation is essential for product quality and process efficiency. Conventional measurement methods, however, are invasive and labour-intensive. This study developed artificial neural network (ANN) models to predict pH and titratable acidity during yoghurt fermentation using CIELAB colour parameters (L, a*, b*). Reconstituted milk powder with 12% total solids was prepared with varying protein levels (4.2–4.8%), inoculum concentrations (1–3%), and fermentation temperatures (36–44 °C). Data were collected every 10 min until pH 4.6 was reached. Forty models were trained for each output variable, using 90% of the data for training and 10% for validation. The first two phases of the fermentation process were clearly distinguishable, lasting between 4.5 and 7 h and exceeding 0.6% lactic acid in all treatments evaluated. The best pH model used two hidden layers with 28 neurons (R2 = 0.969; RMSE = 0.007), while the optimal acidity model had four hidden layers with 32 neurons (R2 = 0.868; RMSE = 0.002). The strong correlation between colour and physicochemical changes confirms the feasibility of this non-destructive approach. Integrating ANN models and colourimetry offers a practical solution for real-time monitoring, helping improve process control in industrial yoghurt production.This research was supported by the Consejo Nacional de Ciencia, Tecnolog\u00EDa e Innovaci\u00F3n Tecnol\u00F3gica (CONCYTEC) and the Programa Nacional de Investigaci\u00F3n Cient\u00EDfica y Estudios Avanzados (PROCIENCIA), under Contrato N\u00B0 PE501082973-2023.Article10.3390/dairy60400412-s2.0-105014521127https://hdl.handle.net/20.500.15037/2371enDairyDairy2624-862XPE501082973-2023PE501082973-2023Multidisciplinary Digital Publishing Institute (MDPI)2025-08-012026-06-27T00:58:54Z2026-06-27T00:58:54Z
spellingShingle fermentation
pH
acidity
CIELAB colour space
artificial neural networks
Alvarado, Ulises
Tacuri, Jhon
Coloma, Alejandro
Gallegos Rojas, Edgar
Callo, Herbert
Valencia-Sullca, Cristina
Curasi Rafael, Nancy
Castillo, Manuel Z.
Development of a Hybrid System Based on the CIELAB Colour Space and Artificial Neural Networks for Monitoring pH and Acidity During Yogurt Fermentation
title Development of a Hybrid System Based on the CIELAB Colour Space and Artificial Neural Networks for Monitoring pH and Acidity During Yogurt Fermentation
title_full Development of a Hybrid System Based on the CIELAB Colour Space and Artificial Neural Networks for Monitoring pH and Acidity During Yogurt Fermentation
title_fullStr Development of a Hybrid System Based on the CIELAB Colour Space and Artificial Neural Networks for Monitoring pH and Acidity During Yogurt Fermentation
title_full_unstemmed Development of a Hybrid System Based on the CIELAB Colour Space and Artificial Neural Networks for Monitoring pH and Acidity During Yogurt Fermentation
title_short Development of a Hybrid System Based on the CIELAB Colour Space and Artificial Neural Networks for Monitoring pH and Acidity During Yogurt Fermentation
title_sort development of a hybrid system based on the cielab colour space and artificial neural networks for monitoring ph and acidity during yogurt fermentation
topic fermentation
pH
acidity
CIELAB colour space
artificial neural networks
topic_facet fermentation
pH
acidity
CIELAB colour space
artificial neural networks
url https://hdl.handle.net/20.500.15037/2371
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