A Multiplex Serological Assay for the Detection of Antibody Responses to Arboviruses
Arthropod borne viruses (arboviruses) are a group of mosquito-borne pathogens that are globally distributed. Since the 2016 outbreak of Zika virus (ZIKV) in the Americas that brought these infections to public awareness, arboviruses continue to impose a considerable public health burden. Their emerg...
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2025
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| author | Marie-Fabrice Gasasira Laura García Françoise Donnadieu Stéphane Pelleau Jessica Vanhomwegen Lova Tsikiniaina Rasoloharimanana Ilaria Dorigatti Matthieu Schoenhals Aïssatou Touré-Baldé Inès Vigan-Womas Makhtar Niang Michaël White |
| author_browse | Aïssatou Touré-Baldé Françoise Donnadieu Ilaria Dorigatti Inès Vigan-Womas Jessica Vanhomwegen Laura García Lova Tsikiniaina Rasoloharimanana Makhtar Niang Marie-Fabrice Gasasira Matthieu Schoenhals Michaël White Stéphane Pelleau |
| author_facet | Marie-Fabrice Gasasira Laura García Françoise Donnadieu Stéphane Pelleau Jessica Vanhomwegen Lova Tsikiniaina Rasoloharimanana Ilaria Dorigatti Matthieu Schoenhals Aïssatou Touré-Baldé Inès Vigan-Womas Makhtar Niang Michaël White |
| author_sort | Marie-Fabrice Gasasira |
| collection | Producción científica |
| description | Arthropod borne viruses (arboviruses) are a group of mosquito-borne pathogens that are globally distributed. Since the 2016 outbreak of Zika virus (ZIKV) in the Americas that brought these infections to public awareness, arboviruses continue to impose a considerable public health burden. Their emergence in previously non-endemic regions is a cause of public health concern. The surveillance and control of arboviral infections is a current priority for the World Health Organization (WHO), which launched a global strategic plan to fight arboviral diseases in 2024. The multiplex® technology has revolutionized the field of serological surveillance, allowing simultaneous measurement of multiple antibodies while requiring minimum sample volume. Building on expertise in the development of multiplex serological tests, a bead-based multiplex assay for the detection of antibodies to arbovirus antigens was developed. The assay comprises multiple antigens specific to common arboviruses, including dengue (DENV), yellow fever (YFV), Zika, and chikungunya (CHIKV) viruses. A semi-automated method was used to covalently bind antigens to microsphere beads. This new protocol is highly effective, enabling the coupling of up to 96 antigens in a single experiment while ensuring reproducible results. Median Fluorescence Intensity (MFI) data were acquired using a user-friendly fluorescence imaging system.When applied to human serum samples from endemic countries, we anticipate that this assay will constitute a powerful tool for sero-surveillance efforts. |
| format | Article |
| id | oai:cris.upeu.edu.pe:20.500.15037/1729 |
| institution | UPeU |
| language | English |
| publishDate | 2025 |
| publishDateRange | 2025 |
| publishDateSort | 2025 |
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| spelling | oai:cris.upeu.edu.pe:20.500.15037/17292026-10-30T16:16:36ZA Multiplex Serological Assay for the Detection of Antibody Responses to ArbovirusesMarie-Fabrice GasasiraLaura GarcíaFrançoise DonnadieuStéphane PelleauJessica VanhomwegenLova Tsikiniaina RasoloharimananaIlaria DorigattiMatthieu SchoenhalsAïssatou Touré-BaldéInès Vigan-WomasMakhtar NiangMichaël WhiteChikungunyaMultiplexSerologyArbovirusVirologyDengue feverAntigenOutbreakBiologyAntibodyDengue virusArbovirus InfectionsAlphavirusVirusImmunologyAntibody responseMultiplex polymerase chain reactionZika virusPublic healthAedesChikungunyaMultiplexSerologyArbovirusDengue feverAntigenOutbreakAntibodyZika Virus immunologyZika Virus immunologyZika Virus immunologyZika Virus immunologyZika Virus immunologyAntibodies, Viral bloodAntibodies, Viral bloodAntibodies, Viral bloodAntibodies, Viral bloodAntibodies, Viral bloodAntibodies, Viral immunologyAntibodies, Viral immunologyAntibodies, Viral immunologyAntibodies, Viral immunologyAntibodies, Viral immunologyAntigens, Viral immunologyAntigens, Viral immunologyAntigens, Viral immunologyAntigens, Viral immunologyAntigens, Viral immunologyArbovirus Infections bloodArbovirus Infections bloodArbovirus Infections bloodArbovirus Infections bloodArbovirus Infections bloodArbovirus Infections diagnosisArbovirus Infections diagnosisArbovirus Infections diagnosisArbovirus Infections diagnosisArbovirus Infections diagnosisArbovirus Infections immunologyArbovirus Infections immunologyArbovirus Infections immunologyArbovirus Infections immunologyArbovirus Infections immunologyArbovirus Infections virologyArbovirus Infections virologyArbovirus Infections virologyArbovirus Infections virologyArbovirus Infections virologyArboviruses immunologyArboviruses immunologyArboviruses immunologyArboviruses immunologyArboviruses immunologyHumansHumansHumansHumansHumansSerologic Tests methodsSerologic Tests methodsSerologic Tests methodsSerologic Tests methodsSerologic Tests methodsHealth Sciences Medicine Public Health, Environmental and Occupational Health Mosquito-borne diseases and controlPhysical Sciences Engineering Biomedical Engineering Biosensors and Analytical DetectionLife Sciences Biochemistry, Genetics and Molecular Biology Molecular Biology Advanced Biosensing Techniques and ApplicationsArthropod borne viruses (arboviruses) are a group of mosquito-borne pathogens that are globally distributed. Since the 2016 outbreak of Zika virus (ZIKV) in the Americas that brought these infections to public awareness, arboviruses continue to impose a considerable public health burden. Their emergence in previously non-endemic regions is a cause of public health concern. The surveillance and control of arboviral infections is a current priority for the World Health Organization (WHO), which launched a global strategic plan to fight arboviral diseases in 2024. The multiplex® technology has revolutionized the field of serological surveillance, allowing simultaneous measurement of multiple antibodies while requiring minimum sample volume. Building on expertise in the development of multiplex serological tests, a bead-based multiplex assay for the detection of antibodies to arbovirus antigens was developed. The assay comprises multiple antigens specific to common arboviruses, including dengue (DENV), yellow fever (YFV), Zika, and chikungunya (CHIKV) viruses. A semi-automated method was used to covalently bind antigens to microsphere beads. This new protocol is highly effective, enabling the coupling of up to 96 antigens in a single experiment while ensuring reproducible results. Median Fluorescence Intensity (MFI) data were acquired using a user-friendly fluorescence imaging system.When applied to human serum samples from endemic countries, we anticipate that this assay will constitute a powerful tool for sero-surveillance efforts.Articlehttps://pubmed.ncbi.nlm.nih.gov/41284716https://pmc.ncbi.nlm.nih.gov/articles/PMC7618357/10.3791/69259https://hdl.handle.net/20.500.15037/1729W4416738061enJournal of Visualized Experiments1940-087X2025-11-042026-06-13T14:20:00Z2026-06-13T14:20:00Z |
| spellingShingle | Chikungunya Multiplex Serology Arbovirus Virology Dengue fever Antigen Outbreak Biology Antibody Dengue virus Arbovirus Infections Alphavirus Virus Immunology Antibody response Multiplex polymerase chain reaction Zika virus Public health Aedes Chikungunya Multiplex Serology Arbovirus Dengue fever Antigen Outbreak Antibody Zika Virus immunology Zika Virus immunology Zika Virus immunology Zika Virus immunology Zika Virus immunology Antibodies, Viral blood Antibodies, Viral blood Antibodies, Viral blood Antibodies, Viral blood Antibodies, Viral blood Antibodies, Viral immunology Antibodies, Viral immunology Antibodies, Viral immunology Antibodies, Viral immunology Antibodies, Viral immunology Antigens, Viral immunology Antigens, Viral immunology Antigens, Viral immunology Antigens, Viral immunology Antigens, Viral immunology Arbovirus Infections blood Arbovirus Infections blood Arbovirus Infections blood Arbovirus Infections blood Arbovirus Infections blood Arbovirus Infections diagnosis Arbovirus Infections diagnosis Arbovirus Infections diagnosis Arbovirus Infections diagnosis Arbovirus Infections diagnosis Arbovirus Infections immunology Arbovirus Infections immunology Arbovirus Infections immunology Arbovirus Infections immunology Arbovirus Infections immunology Arbovirus Infections virology Arbovirus Infections virology Arbovirus Infections virology Arbovirus Infections virology Arbovirus Infections virology Arboviruses immunology Arboviruses immunology Arboviruses immunology Arboviruses immunology Arboviruses immunology Humans Humans Humans Humans Humans Serologic Tests methods Serologic Tests methods Serologic Tests methods Serologic Tests methods Serologic Tests methods Health Sciences Medicine Public Health, Environmental and Occupational Health Mosquito-borne diseases and control Physical Sciences Engineering Biomedical Engineering Biosensors and Analytical Detection Life Sciences Biochemistry, Genetics and Molecular Biology Molecular Biology Advanced Biosensing Techniques and Applications Marie-Fabrice Gasasira Laura García Françoise Donnadieu Stéphane Pelleau Jessica Vanhomwegen Lova Tsikiniaina Rasoloharimanana Ilaria Dorigatti Matthieu Schoenhals Aïssatou Touré-Baldé Inès Vigan-Womas Makhtar Niang Michaël White A Multiplex Serological Assay for the Detection of Antibody Responses to Arboviruses |
| title | A Multiplex Serological Assay for the Detection of Antibody Responses to Arboviruses |
| title_full | A Multiplex Serological Assay for the Detection of Antibody Responses to Arboviruses |
| title_fullStr | A Multiplex Serological Assay for the Detection of Antibody Responses to Arboviruses |
| title_full_unstemmed | A Multiplex Serological Assay for the Detection of Antibody Responses to Arboviruses |
| title_short | A Multiplex Serological Assay for the Detection of Antibody Responses to Arboviruses |
| title_sort | multiplex serological assay for the detection of antibody responses to arboviruses |
| topic | Chikungunya Multiplex Serology Arbovirus Virology Dengue fever Antigen Outbreak Biology Antibody Dengue virus Arbovirus Infections Alphavirus Virus Immunology Antibody response Multiplex polymerase chain reaction Zika virus Public health Aedes Chikungunya Multiplex Serology Arbovirus Dengue fever Antigen Outbreak Antibody Zika Virus immunology Zika Virus immunology Zika Virus immunology Zika Virus immunology Zika Virus immunology Antibodies, Viral blood Antibodies, Viral blood Antibodies, Viral blood Antibodies, Viral blood Antibodies, Viral blood Antibodies, Viral immunology Antibodies, Viral immunology Antibodies, Viral immunology Antibodies, Viral immunology Antibodies, Viral immunology Antigens, Viral immunology Antigens, Viral immunology Antigens, Viral immunology Antigens, Viral immunology Antigens, Viral immunology Arbovirus Infections blood Arbovirus Infections blood Arbovirus Infections blood Arbovirus Infections blood Arbovirus Infections blood Arbovirus Infections diagnosis Arbovirus Infections diagnosis Arbovirus Infections diagnosis Arbovirus Infections diagnosis Arbovirus Infections diagnosis Arbovirus Infections immunology Arbovirus Infections immunology Arbovirus Infections immunology Arbovirus Infections immunology Arbovirus Infections immunology Arbovirus Infections virology Arbovirus Infections virology Arbovirus Infections virology Arbovirus Infections virology Arbovirus Infections virology Arboviruses immunology Arboviruses immunology Arboviruses immunology Arboviruses immunology Arboviruses immunology Humans Humans Humans Humans Humans Serologic Tests methods Serologic Tests methods Serologic Tests methods Serologic Tests methods Serologic Tests methods Health Sciences Medicine Public Health, Environmental and Occupational Health Mosquito-borne diseases and control Physical Sciences Engineering Biomedical Engineering Biosensors and Analytical Detection Life Sciences Biochemistry, Genetics and Molecular Biology Molecular Biology Advanced Biosensing Techniques and Applications |
| topic_facet | Chikungunya Multiplex Serology Arbovirus Virology Dengue fever Antigen Outbreak Biology Antibody Dengue virus Arbovirus Infections Alphavirus Virus Immunology Antibody response Multiplex polymerase chain reaction Zika virus Public health Aedes Chikungunya Multiplex Serology Arbovirus Dengue fever Antigen Outbreak Antibody Zika Virus immunology Zika Virus immunology Zika Virus immunology Zika Virus immunology Zika Virus immunology Antibodies, Viral blood Antibodies, Viral blood Antibodies, Viral blood Antibodies, Viral blood Antibodies, Viral blood Antibodies, Viral immunology Antibodies, Viral immunology Antibodies, Viral immunology Antibodies, Viral immunology Antibodies, Viral immunology Antigens, Viral immunology Antigens, Viral immunology Antigens, Viral immunology Antigens, Viral immunology Antigens, Viral immunology Arbovirus Infections blood Arbovirus Infections blood Arbovirus Infections blood Arbovirus Infections blood Arbovirus Infections blood Arbovirus Infections diagnosis Arbovirus Infections diagnosis Arbovirus Infections diagnosis Arbovirus Infections diagnosis Arbovirus Infections diagnosis Arbovirus Infections immunology Arbovirus Infections immunology Arbovirus Infections immunology Arbovirus Infections immunology Arbovirus Infections immunology Arbovirus Infections virology Arbovirus Infections virology Arbovirus Infections virology Arbovirus Infections virology Arbovirus Infections virology Arboviruses immunology Arboviruses immunology Arboviruses immunology Arboviruses immunology Arboviruses immunology Humans Humans Humans Humans Humans Serologic Tests methods Serologic Tests methods Serologic Tests methods Serologic Tests methods Serologic Tests methods Health Sciences Medicine Public Health, Environmental and Occupational Health Mosquito-borne diseases and control Physical Sciences Engineering Biomedical Engineering Biosensors and Analytical Detection Life Sciences Biochemistry, Genetics and Molecular Biology Molecular Biology Advanced Biosensing Techniques and Applications |
| url | https://pubmed.ncbi.nlm.nih.gov/41284716 https://pmc.ncbi.nlm.nih.gov/articles/PMC7618357/ https://hdl.handle.net/20.500.15037/1729 |
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