Increased Blood Ethanol Elimination in Rats Treated with Halothane
The effect of chronic halothane inhalation and blood ethanol elimination was studied in the rat. Hepatic α-glycerophosphate, oxidase ADH and malic enzyme activities were determined. Malic enzyme activity was also assayed in adipose tissue. Halothane-treated rats showed an increased ethanol eliminati...
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2008
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| author | G Ugarte María Eugenia Pino T Pereda H Iturriaga |
| author_browse | G Ugarte H Iturriaga María Eugenia Pino T Pereda |
| author_facet | G Ugarte María Eugenia Pino T Pereda H Iturriaga |
| author_sort | G Ugarte |
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| description | The effect of chronic halothane inhalation and blood ethanol elimination was studied in the rat. Hepatic α-glycerophosphate, oxidase ADH and malic enzyme activities were determined. Malic enzyme activity was also assayed in adipose tissue. Halothane-treated rats showed an increased ethanol elimination (378 ± 13 vs. 292 ± 19; p < 0.01). Hepatic and adipose tissue malic enzyme activities were significantly enhanced in halothane-treated rats (6.26 ± 1.2 vs. 1.03 ± 0.23; p < 0.002 for the liver and 1.84 ± 0.71 vs. 0.75 ± 0.33 for adipose tissue). Mitochondrial α-glycerophosphate oxidase activity was also significantly increased in the liver of halothane-treated animals (p < 0.05) while no changes in ADH activity were observed. The enhanced ethanol elimination after halothane treatment, a substance which inhibits microsomal enzyme, is possibly due to increased NADH reoxidation. Another possibility explaining augmented ethanol metabolism may be an increased incorporation of ethanol into lipids related to the enhancement of malic enzyme activity. |
| format | Article |
| id | oai:cris.upeu.edu.pe:20.500.15037/1658 |
| institution | UPeU |
| language | English |
| publishDate | 2008 |
| publishDateRange | 2008 |
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| spelling | oai:cris.upeu.edu.pe:20.500.15037/16582026-10-30T16:16:24ZIncreased Blood Ethanol Elimination in Rats Treated with HalothaneG UgarteMaría Eugenia PinoT PeredaH IturriagaHalothaneEthanolMalic enzymeChemistryAdipose tissueEndocrinologyInternal medicineEnzymeMicrosomeEnzyme assayInhalationEthanol metabolismBiochemistryDehydrogenaseMedicineAnesthesiaOrganic chemistryHalothaneEthanolMalic enzymeChemistryAdipose tissueEndocrinologyInternal medicineEnzymeMicrosomeEnzyme assayInhalationEthanol metabolismBiochemistryDehydrogenaseMedicineAnesthesiaAlcohol Oxidoreductases metabolismAlcohol Oxidoreductases metabolismAlcohol Oxidoreductases metabolismEthanol bloodEthanol bloodEthanol bloodEthanol metabolismEthanol metabolismEthanol metabolismAnimalsAnimalsAnimalsBody WeightBody WeightBody WeightFemaleFemaleFemaleGlycerophosphatesGlycerophosphatesGlycerophosphatesHalothane pharmacologyHalothane pharmacologyHalothane pharmacologyKineticsKineticsKineticsLiver drug effectsLiver drug effectsLiver drug effectsMalate Dehydrogenase metabolismMalate Dehydrogenase metabolismMalate Dehydrogenase metabolismMitochondria, Liver enzymologyMitochondria, Liver enzymologyMitochondria, Liver enzymologyOrgan SizeOrgan SizeOrgan SizeRatsRatsRatsHealth Sciences Medicine Pathology and Forensic Medicine Alcohol Consumption and Health EffectsLife Sciences Biochemistry, Genetics and Molecular Biology Molecular Biology Peroxisome Proliferator-Activated ReceptorsHealth Sciences Medicine Epidemiology Liver Disease Diagnosis and TreatmentThe effect of chronic halothane inhalation and blood ethanol elimination was studied in the rat. Hepatic α-glycerophosphate, oxidase ADH and malic enzyme activities were determined. Malic enzyme activity was also assayed in adipose tissue. Halothane-treated rats showed an increased ethanol elimination (378 ± 13 vs. 292 ± 19; p < 0.01). Hepatic and adipose tissue malic enzyme activities were significantly enhanced in halothane-treated rats (6.26 ± 1.2 vs. 1.03 ± 0.23; p < 0.002 for the liver and 1.84 ± 0.71 vs. 0.75 ± 0.33 for adipose tissue). Mitochondrial α-glycerophosphate oxidase activity was also significantly increased in the liver of halothane-treated animals (p < 0.05) while no changes in ADH activity were observed. The enhanced ethanol elimination after halothane treatment, a substance which inhibits microsomal enzyme, is possibly due to increased NADH reoxidation. Another possibility explaining augmented ethanol metabolism may be an increased incorporation of ethanol into lipids related to the enhancement of malic enzyme activity.Articlehttps://pubmed.ncbi.nlm.nih.gov/4728040https://repositorio.uchile.cl/handle/2250/16222510.1159/000136396https://hdl.handle.net/20.500.15037/1658W2062307127enPharmacology0031-70121423-03132008-05-282026-06-13T14:18:00Z2026-06-13T14:18:00Z |
| spellingShingle | Halothane Ethanol Malic enzyme Chemistry Adipose tissue Endocrinology Internal medicine Enzyme Microsome Enzyme assay Inhalation Ethanol metabolism Biochemistry Dehydrogenase Medicine Anesthesia Organic chemistry Halothane Ethanol Malic enzyme Chemistry Adipose tissue Endocrinology Internal medicine Enzyme Microsome Enzyme assay Inhalation Ethanol metabolism Biochemistry Dehydrogenase Medicine Anesthesia Alcohol Oxidoreductases metabolism Alcohol Oxidoreductases metabolism Alcohol Oxidoreductases metabolism Ethanol blood Ethanol blood Ethanol blood Ethanol metabolism Ethanol metabolism Ethanol metabolism Animals Animals Animals Body Weight Body Weight Body Weight Female Female Female Glycerophosphates Glycerophosphates Glycerophosphates Halothane pharmacology Halothane pharmacology Halothane pharmacology Kinetics Kinetics Kinetics Liver drug effects Liver drug effects Liver drug effects Malate Dehydrogenase metabolism Malate Dehydrogenase metabolism Malate Dehydrogenase metabolism Mitochondria, Liver enzymology Mitochondria, Liver enzymology Mitochondria, Liver enzymology Organ Size Organ Size Organ Size Rats Rats Rats Health Sciences Medicine Pathology and Forensic Medicine Alcohol Consumption and Health Effects Life Sciences Biochemistry, Genetics and Molecular Biology Molecular Biology Peroxisome Proliferator-Activated Receptors Health Sciences Medicine Epidemiology Liver Disease Diagnosis and Treatment G Ugarte María Eugenia Pino T Pereda H Iturriaga Increased Blood Ethanol Elimination in Rats Treated with Halothane |
| title | Increased Blood Ethanol Elimination in Rats Treated with Halothane |
| title_full | Increased Blood Ethanol Elimination in Rats Treated with Halothane |
| title_fullStr | Increased Blood Ethanol Elimination in Rats Treated with Halothane |
| title_full_unstemmed | Increased Blood Ethanol Elimination in Rats Treated with Halothane |
| title_short | Increased Blood Ethanol Elimination in Rats Treated with Halothane |
| title_sort | increased blood ethanol elimination in rats treated with halothane |
| topic | Halothane Ethanol Malic enzyme Chemistry Adipose tissue Endocrinology Internal medicine Enzyme Microsome Enzyme assay Inhalation Ethanol metabolism Biochemistry Dehydrogenase Medicine Anesthesia Organic chemistry Halothane Ethanol Malic enzyme Chemistry Adipose tissue Endocrinology Internal medicine Enzyme Microsome Enzyme assay Inhalation Ethanol metabolism Biochemistry Dehydrogenase Medicine Anesthesia Alcohol Oxidoreductases metabolism Alcohol Oxidoreductases metabolism Alcohol Oxidoreductases metabolism Ethanol blood Ethanol blood Ethanol blood Ethanol metabolism Ethanol metabolism Ethanol metabolism Animals Animals Animals Body Weight Body Weight Body Weight Female Female Female Glycerophosphates Glycerophosphates Glycerophosphates Halothane pharmacology Halothane pharmacology Halothane pharmacology Kinetics Kinetics Kinetics Liver drug effects Liver drug effects Liver drug effects Malate Dehydrogenase metabolism Malate Dehydrogenase metabolism Malate Dehydrogenase metabolism Mitochondria, Liver enzymology Mitochondria, Liver enzymology Mitochondria, Liver enzymology Organ Size Organ Size Organ Size Rats Rats Rats Health Sciences Medicine Pathology and Forensic Medicine Alcohol Consumption and Health Effects Life Sciences Biochemistry, Genetics and Molecular Biology Molecular Biology Peroxisome Proliferator-Activated Receptors Health Sciences Medicine Epidemiology Liver Disease Diagnosis and Treatment |
| topic_facet | Halothane Ethanol Malic enzyme Chemistry Adipose tissue Endocrinology Internal medicine Enzyme Microsome Enzyme assay Inhalation Ethanol metabolism Biochemistry Dehydrogenase Medicine Anesthesia Organic chemistry Halothane Ethanol Malic enzyme Chemistry Adipose tissue Endocrinology Internal medicine Enzyme Microsome Enzyme assay Inhalation Ethanol metabolism Biochemistry Dehydrogenase Medicine Anesthesia Alcohol Oxidoreductases metabolism Alcohol Oxidoreductases metabolism Alcohol Oxidoreductases metabolism Ethanol blood Ethanol blood Ethanol blood Ethanol metabolism Ethanol metabolism Ethanol metabolism Animals Animals Animals Body Weight Body Weight Body Weight Female Female Female Glycerophosphates Glycerophosphates Glycerophosphates Halothane pharmacology Halothane pharmacology Halothane pharmacology Kinetics Kinetics Kinetics Liver drug effects Liver drug effects Liver drug effects Malate Dehydrogenase metabolism Malate Dehydrogenase metabolism Malate Dehydrogenase metabolism Mitochondria, Liver enzymology Mitochondria, Liver enzymology Mitochondria, Liver enzymology Organ Size Organ Size Organ Size Rats Rats Rats Health Sciences Medicine Pathology and Forensic Medicine Alcohol Consumption and Health Effects Life Sciences Biochemistry, Genetics and Molecular Biology Molecular Biology Peroxisome Proliferator-Activated Receptors Health Sciences Medicine Epidemiology Liver Disease Diagnosis and Treatment |
| url | https://pubmed.ncbi.nlm.nih.gov/4728040 https://repositorio.uchile.cl/handle/2250/162225 https://hdl.handle.net/20.500.15037/1658 |
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