Blastulation time measured with time-lapse system can predict in vitro viability of bovine blastocysts
The objective of this study was to evaluate the time of blastulation monitored by time-lapse technology to predict in vitro viability of bovine blastocysts. This technology can be a powerful tool for bovine embryos selection with higher implantation capacity and competence. Also, in humans an early...
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2023
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| author | Carmen Huayhua Misael Rodríguez Jhorjhi Vega Mario Briones L. Rodríguez-Álvarez E. Mellisho |
| author_browse | Carmen Huayhua E. Mellisho Jhorjhi Vega L. Rodríguez-Álvarez Mario Briones Misael Rodríguez |
| author_facet | Carmen Huayhua Misael Rodríguez Jhorjhi Vega Mario Briones L. Rodríguez-Álvarez E. Mellisho |
| author_sort | Carmen Huayhua |
| collection | Producción científica |
| description | The objective of this study was to evaluate the time of blastulation monitored by time-lapse technology to predict in vitro viability of bovine blastocysts. This technology can be a powerful tool for bovine embryos selection with higher implantation capacity and competence. Also, in humans an early blastulation is associated with higher quality and pregnancy rate. Cumulus oocyte complexes (COCs) were matured for 20 to 22 h and then fertilized by co-incubation of COCs and spermatozoa (10,000 sperm per oocyte) for 18 h. Presumptive zygotes were placed individually in microwells, in droplets of commercial culture medium. The Primo Vision TL system (EVO+; Vitrolife) captured digital images of developing embryos every 15 minutes. The time frame from IVF to the start of blastulation (tSB) and to blastocyst development (tB) was recorded. After day 7.5, the blastocysts were in vitro culture for 48 h until day 9.5 after IVF to evaluate post hatching development. In vitro viability was evaluated at day 9.5: those with a diameter greater than 200 μm and a total cell count greater than 180 were classified as viable (value 1), while the rest were classified as non in vitro viable (value 0). The area under the ROC curve (AUC) was estimated to determine the predictive power of in vitro viability through blastulation time. In addition, binary logistic regression analysis was used to generate a mathematical model with morphokinetic variables that allow the best prediction of in vitro viability. In 13 sessions, the blastocyst production rate was 46.2% (96/208). The cut-off time to discriminate early or late blastulation was 149.8 h. The post-hatching development of the embryos with early blastulation was 63.3% (31/49), being statistically superior (p = 0.001) than the late blastulation group 14.9% (7/47). Likewise, the time of blastulation showed an accuracy of 90.8% (p < 0.001) in predicting in vitro viability of bovine blastocysts. In conclusion, the selection of blastocysts based on blastulation time (< 155 h) and blastocyst diameter measured on day 7.5 after IVF (> 180 μm) maximizes the in vitro viability. |
| format | Article |
| id | oai:cris.upeu.edu.pe:20.500.15037/1366 |
| institution | UPeU |
| language | English |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
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| spelling | oai:cris.upeu.edu.pe:20.500.15037/13662026-10-30T16:15:40ZBlastulation time measured with time-lapse system can predict in vitro viability of bovine blastocystsCarmen HuayhuaMisael RodríguezJhorjhi VegaMario BrionesL. Rodríguez-ÁlvarezE. MellishoBlastulaBlastocystAndrologyBiologyCryopreservationIn vitro maturationOocyteIn vitro fertilisationEmbryo cultureZygoteEmbryoEmbryogenesisMedicineGeneticsGastrulationBlastulaBlastocystAndrologyBiologyCryopreservationIn vitro maturationOocyteIn vitro fertilisationEmbryo cultureZygoteEmbryoEmbryogenesisMedicineGeneticsAnimalsAnimalsAnimalsAnimalsBlastocystBlastocystBlastocystBlastocystCattleCattleCattleCattleFemaleFemaleFemaleFemaleFertilization in Vitro veterinaryFertilization in Vitro veterinaryFertilization in Vitro veterinaryFertilization in Vitro veterinaryHumansHumansHumansHumansMaleMaleMaleMalePregnancyPregnancyPregnancyPregnancySemenSemenSemenSemenEmbryo Culture TechniquesEmbryo Culture TechniquesEmbryo Culture TechniquesEmbryo Culture TechniquesEmbryonic DevelopmentEmbryonic DevelopmentEmbryonic DevelopmentEmbryonic DevelopmentTime-Lapse ImagingTime-Lapse ImagingTime-Lapse ImagingTime-Lapse ImagingHealth Sciences Medicine Public Health, Environmental and Occupational Health Reproductive Biology and FertilityHealth Sciences Medicine Reproductive Medicine Reproductive Health and TechnologiesHealth Sciences Medicine Reproductive Medicine Sperm and Testicular FunctionThe objective of this study was to evaluate the time of blastulation monitored by time-lapse technology to predict in vitro viability of bovine blastocysts. This technology can be a powerful tool for bovine embryos selection with higher implantation capacity and competence. Also, in humans an early blastulation is associated with higher quality and pregnancy rate. Cumulus oocyte complexes (COCs) were matured for 20 to 22 h and then fertilized by co-incubation of COCs and spermatozoa (10,000 sperm per oocyte) for 18 h. Presumptive zygotes were placed individually in microwells, in droplets of commercial culture medium. The Primo Vision TL system (EVO+; Vitrolife) captured digital images of developing embryos every 15 minutes. The time frame from IVF to the start of blastulation (tSB) and to blastocyst development (tB) was recorded. After day 7.5, the blastocysts were in vitro culture for 48 h until day 9.5 after IVF to evaluate post hatching development. In vitro viability was evaluated at day 9.5: those with a diameter greater than 200 μm and a total cell count greater than 180 were classified as viable (value 1), while the rest were classified as non in vitro viable (value 0). The area under the ROC curve (AUC) was estimated to determine the predictive power of in vitro viability through blastulation time. In addition, binary logistic regression analysis was used to generate a mathematical model with morphokinetic variables that allow the best prediction of in vitro viability. In 13 sessions, the blastocyst production rate was 46.2% (96/208). The cut-off time to discriminate early or late blastulation was 149.8 h. The post-hatching development of the embryos with early blastulation was 63.3% (31/49), being statistically superior (p = 0.001) than the late blastulation group 14.9% (7/47). Likewise, the time of blastulation showed an accuracy of 90.8% (p < 0.001) in predicting in vitro viability of bovine blastocysts. In conclusion, the selection of blastocysts based on blastulation time (< 155 h) and blastocyst diameter measured on day 7.5 after IVF (> 180 μm) maximizes the in vitro viability.Articlehttps://pubmed.ncbi.nlm.nih.gov/37561791https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0289751&type=printablehttps://doi.org/10.1371/journal.pone.028975110.1371/journal.pone.0289751https://hdl.handle.net/20.500.15037/1366W4385727370enPLoS ONE1932-62032023-08-102026-06-13T09:55:41Z2026-06-13T09:55:41Z |
| spellingShingle | Blastula Blastocyst Andrology Biology Cryopreservation In vitro maturation Oocyte In vitro fertilisation Embryo culture Zygote Embryo Embryogenesis Medicine Genetics Gastrulation Blastula Blastocyst Andrology Biology Cryopreservation In vitro maturation Oocyte In vitro fertilisation Embryo culture Zygote Embryo Embryogenesis Medicine Genetics Animals Animals Animals Animals Blastocyst Blastocyst Blastocyst Blastocyst Cattle Cattle Cattle Cattle Female Female Female Female Fertilization in Vitro veterinary Fertilization in Vitro veterinary Fertilization in Vitro veterinary Fertilization in Vitro veterinary Humans Humans Humans Humans Male Male Male Male Pregnancy Pregnancy Pregnancy Pregnancy Semen Semen Semen Semen Embryo Culture Techniques Embryo Culture Techniques Embryo Culture Techniques Embryo Culture Techniques Embryonic Development Embryonic Development Embryonic Development Embryonic Development Time-Lapse Imaging Time-Lapse Imaging Time-Lapse Imaging Time-Lapse Imaging Health Sciences Medicine Public Health, Environmental and Occupational Health Reproductive Biology and Fertility Health Sciences Medicine Reproductive Medicine Reproductive Health and Technologies Health Sciences Medicine Reproductive Medicine Sperm and Testicular Function Carmen Huayhua Misael Rodríguez Jhorjhi Vega Mario Briones L. Rodríguez-Álvarez E. Mellisho Blastulation time measured with time-lapse system can predict in vitro viability of bovine blastocysts |
| title | Blastulation time measured with time-lapse system can predict in vitro viability of bovine blastocysts |
| title_full | Blastulation time measured with time-lapse system can predict in vitro viability of bovine blastocysts |
| title_fullStr | Blastulation time measured with time-lapse system can predict in vitro viability of bovine blastocysts |
| title_full_unstemmed | Blastulation time measured with time-lapse system can predict in vitro viability of bovine blastocysts |
| title_short | Blastulation time measured with time-lapse system can predict in vitro viability of bovine blastocysts |
| title_sort | blastulation time measured with time lapse system can predict in vitro viability of bovine blastocysts |
| topic | Blastula Blastocyst Andrology Biology Cryopreservation In vitro maturation Oocyte In vitro fertilisation Embryo culture Zygote Embryo Embryogenesis Medicine Genetics Gastrulation Blastula Blastocyst Andrology Biology Cryopreservation In vitro maturation Oocyte In vitro fertilisation Embryo culture Zygote Embryo Embryogenesis Medicine Genetics Animals Animals Animals Animals Blastocyst Blastocyst Blastocyst Blastocyst Cattle Cattle Cattle Cattle Female Female Female Female Fertilization in Vitro veterinary Fertilization in Vitro veterinary Fertilization in Vitro veterinary Fertilization in Vitro veterinary Humans Humans Humans Humans Male Male Male Male Pregnancy Pregnancy Pregnancy Pregnancy Semen Semen Semen Semen Embryo Culture Techniques Embryo Culture Techniques Embryo Culture Techniques Embryo Culture Techniques Embryonic Development Embryonic Development Embryonic Development Embryonic Development Time-Lapse Imaging Time-Lapse Imaging Time-Lapse Imaging Time-Lapse Imaging Health Sciences Medicine Public Health, Environmental and Occupational Health Reproductive Biology and Fertility Health Sciences Medicine Reproductive Medicine Reproductive Health and Technologies Health Sciences Medicine Reproductive Medicine Sperm and Testicular Function |
| topic_facet | Blastula Blastocyst Andrology Biology Cryopreservation In vitro maturation Oocyte In vitro fertilisation Embryo culture Zygote Embryo Embryogenesis Medicine Genetics Gastrulation Blastula Blastocyst Andrology Biology Cryopreservation In vitro maturation Oocyte In vitro fertilisation Embryo culture Zygote Embryo Embryogenesis Medicine Genetics Animals Animals Animals Animals Blastocyst Blastocyst Blastocyst Blastocyst Cattle Cattle Cattle Cattle Female Female Female Female Fertilization in Vitro veterinary Fertilization in Vitro veterinary Fertilization in Vitro veterinary Fertilization in Vitro veterinary Humans Humans Humans Humans Male Male Male Male Pregnancy Pregnancy Pregnancy Pregnancy Semen Semen Semen Semen Embryo Culture Techniques Embryo Culture Techniques Embryo Culture Techniques Embryo Culture Techniques Embryonic Development Embryonic Development Embryonic Development Embryonic Development Time-Lapse Imaging Time-Lapse Imaging Time-Lapse Imaging Time-Lapse Imaging Health Sciences Medicine Public Health, Environmental and Occupational Health Reproductive Biology and Fertility Health Sciences Medicine Reproductive Medicine Reproductive Health and Technologies Health Sciences Medicine Reproductive Medicine Sperm and Testicular Function |
| url | https://pubmed.ncbi.nlm.nih.gov/37561791 https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0289751&type=printable https://doi.org/10.1371/journal.pone.0289751 https://hdl.handle.net/20.500.15037/1366 |
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