A simple method for the determination of fluoroquinolone residues in tilapia (Oreochromis niloticus) and pacu (Piaractus mesopotamicus) employing LC-MS/MS QToF
The use of antimicrobials in livestock production is a powerful resource applied throughout the world to guarantee high yield and control bacterial diseases in aquaculture. However, residues of these substances in animal products represent a potential risk to consumer health when residue levels are...
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| Formato: | Artículo |
| Lenguaje: | inglés |
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2013
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| _version_ | 1881508844850380800 |
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| author | Silvia Pilco-Quesada Jonas Augusto Rizzato Paschoal Félix Guillermo Reyes Reyes |
| author_browse | Félix Guillermo Reyes Reyes Jonas Augusto Rizzato Paschoal Silvia Pilco-Quesada |
| author_facet | Silvia Pilco-Quesada Jonas Augusto Rizzato Paschoal Félix Guillermo Reyes Reyes |
| author_sort | Silvia Pilco-Quesada |
| collection | Producción científica |
| description | The use of antimicrobials in livestock production is a powerful resource applied throughout the world to guarantee high yield and control bacterial diseases in aquaculture. However, residues of these substances in animal products represent a potential risk to consumer health when residue levels are above the established maximum residue limits (MRLs). Fluoroquinolones (FQs) are antimicrobials commonly used worldwide in aquaculture. The aim of this work was to develop and validate a simple analytical method for the simultaneous determination of norfloxacin, danofloxacin, enrofloxacin and ciprofloxacin levels in tilapia (Oreochromis niloticus) and pacu (Piaractus mesopotamicus) fillets using liquid chromatography–tandem mass spectrometry (LC-MS/MS) quadrupole time of flight (QToF). The FQs were extracted from the fillets with 1% acetic acid–methanol and 1% acetic acid–acetonitrile solutions using ultrasonic assistance. The clean-up was performed with hexane. Chromatographic separation was conducted in an XTerra RP18 column (2.1 × 150 mm, 5 µm) at 25 °C with a flow of 0.2 mL min−1. The mobile phase consisted of 0.1% aqueous formic acid and acetonitrile, with gradient elution. The validation parameters for all FQs were linearity (>0.99), intra-day precision (CV of 1%–9%), inter-day precision (CV of 3%–17%), decision limit (63–126 ng g−1), detection capability (76 –152 ng g−1) and accuracy (90%–111%). The limit of quantification was lower than the MRL for each FQ, indicating that the method is suitable for the determination of the FQ levels in the fish fillets. The mass analyser of the QToF type was able to confirm the identities of the FQs with an error of the accuracy of the mass (reasons m/z) of less than 10 ppm. |
| format | Article |
| id | oai:cris.upeu.edu.pe:20.500.15037/1210 |
| institution | UPeU |
| language | English |
| publishDate | 2013 |
| publishDateRange | 2013 |
| publishDateSort | 2013 |
| record_format | dspace |
| spelling | oai:cris.upeu.edu.pe:20.500.15037/12102026-10-30T16:15:22ZA simple method for the determination of fluoroquinolone residues in tilapia (Oreochromis niloticus) and pacu (Piaractus mesopotamicus) employing LC-MS/MS QToFSilvia Pilco-QuesadaJonas Augusto Rizzato PaschoalFélix Guillermo Reyes ReyesPiaractus mesopotamicusPacuOreochromisTilapiaChromatographyFisheryChemistryFish <Actinopterygii>BiologyPiaractus mesopotamicusPacuOreochromisTilapiaChromatographyFisheryChemistryFish <Actinopterygii>BiologyLife Sciences Agricultural and Biological Sciences Food Science Pesticide Residue Analysis and SafetyHealth Sciences Medicine Pharmacology Antibiotics Pharmacokinetics and EfficacyLife Sciences Agricultural and Biological Sciences Animal Science and Zoology Pharmacological Effects and AssaysThe use of antimicrobials in livestock production is a powerful resource applied throughout the world to guarantee high yield and control bacterial diseases in aquaculture. However, residues of these substances in animal products represent a potential risk to consumer health when residue levels are above the established maximum residue limits (MRLs). Fluoroquinolones (FQs) are antimicrobials commonly used worldwide in aquaculture. The aim of this work was to develop and validate a simple analytical method for the simultaneous determination of norfloxacin, danofloxacin, enrofloxacin and ciprofloxacin levels in tilapia (Oreochromis niloticus) and pacu (Piaractus mesopotamicus) fillets using liquid chromatography–tandem mass spectrometry (LC-MS/MS) quadrupole time of flight (QToF). The FQs were extracted from the fillets with 1% acetic acid–methanol and 1% acetic acid–acetonitrile solutions using ultrasonic assistance. The clean-up was performed with hexane. Chromatographic separation was conducted in an XTerra RP18 column (2.1 × 150 mm, 5 µm) at 25 °C with a flow of 0.2 mL min−1. The mobile phase consisted of 0.1% aqueous formic acid and acetonitrile, with gradient elution. The validation parameters for all FQs were linearity (>0.99), intra-day precision (CV of 1%–9%), inter-day precision (CV of 3%–17%), decision limit (63–126 ng g−1), detection capability (76 –152 ng g−1) and accuracy (90%–111%). The limit of quantification was lower than the MRL for each FQ, indicating that the method is suitable for the determination of the FQ levels in the fish fillets. The mass analyser of the QToF type was able to confirm the identities of the FQs with an error of the accuracy of the mass (reasons m/z) of less than 10 ppm.Article10.1080/19440049.2013.791750https://hdl.handle.net/20.500.15037/1210W1978583172enFood Additives & Contaminants Part A1944-00491944-00572013-04-222026-06-13T09:50:08Z2026-06-13T09:50:08Z |
| spellingShingle | Piaractus mesopotamicus Pacu Oreochromis Tilapia Chromatography Fishery Chemistry Fish <Actinopterygii> Biology Piaractus mesopotamicus Pacu Oreochromis Tilapia Chromatography Fishery Chemistry Fish <Actinopterygii> Biology Life Sciences Agricultural and Biological Sciences Food Science Pesticide Residue Analysis and Safety Health Sciences Medicine Pharmacology Antibiotics Pharmacokinetics and Efficacy Life Sciences Agricultural and Biological Sciences Animal Science and Zoology Pharmacological Effects and Assays Silvia Pilco-Quesada Jonas Augusto Rizzato Paschoal Félix Guillermo Reyes Reyes A simple method for the determination of fluoroquinolone residues in tilapia (Oreochromis niloticus) and pacu (Piaractus mesopotamicus) employing LC-MS/MS QToF |
| title | A simple method for the determination of fluoroquinolone residues in tilapia (Oreochromis niloticus) and pacu (Piaractus mesopotamicus) employing LC-MS/MS QToF |
| title_full | A simple method for the determination of fluoroquinolone residues in tilapia (Oreochromis niloticus) and pacu (Piaractus mesopotamicus) employing LC-MS/MS QToF |
| title_fullStr | A simple method for the determination of fluoroquinolone residues in tilapia (Oreochromis niloticus) and pacu (Piaractus mesopotamicus) employing LC-MS/MS QToF |
| title_full_unstemmed | A simple method for the determination of fluoroquinolone residues in tilapia (Oreochromis niloticus) and pacu (Piaractus mesopotamicus) employing LC-MS/MS QToF |
| title_short | A simple method for the determination of fluoroquinolone residues in tilapia (Oreochromis niloticus) and pacu (Piaractus mesopotamicus) employing LC-MS/MS QToF |
| title_sort | simple method for the determination of fluoroquinolone residues in tilapia oreochromis niloticus and pacu piaractus mesopotamicus employing lc ms ms qtof |
| topic | Piaractus mesopotamicus Pacu Oreochromis Tilapia Chromatography Fishery Chemistry Fish <Actinopterygii> Biology Piaractus mesopotamicus Pacu Oreochromis Tilapia Chromatography Fishery Chemistry Fish <Actinopterygii> Biology Life Sciences Agricultural and Biological Sciences Food Science Pesticide Residue Analysis and Safety Health Sciences Medicine Pharmacology Antibiotics Pharmacokinetics and Efficacy Life Sciences Agricultural and Biological Sciences Animal Science and Zoology Pharmacological Effects and Assays |
| topic_facet | Piaractus mesopotamicus Pacu Oreochromis Tilapia Chromatography Fishery Chemistry Fish <Actinopterygii> Biology Piaractus mesopotamicus Pacu Oreochromis Tilapia Chromatography Fishery Chemistry Fish <Actinopterygii> Biology Life Sciences Agricultural and Biological Sciences Food Science Pesticide Residue Analysis and Safety Health Sciences Medicine Pharmacology Antibiotics Pharmacokinetics and Efficacy Life Sciences Agricultural and Biological Sciences Animal Science and Zoology Pharmacological Effects and Assays |
| url | https://hdl.handle.net/20.500.15037/1210 |
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