Expresión diferencial de micro-ARN circulantes en pacientes con tuberculosis activa y latente
OBJECTIVES: To analyze the differential expression of miR-21, miR-29a, miR-99b and miR-155 in serum samples from patients with latent tuberculosis (TB) and active TB compared to healthy controls. MATE RIALS AND METHODS: We used 28 serum samples (9 with active TB, 10 with latent TB and 9 healthy con...
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2020
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| author | José Yareta-Yareta Marco Galarza Silvia Capristano Oscar Pellón-Cárdenas César Sánchez Jorge Ballon Heinner Guio |
| author_browse | César Sánchez Heinner Guio Jorge Ballon José Yareta-Yareta Marco Galarza Oscar Pellón-Cárdenas Silvia Capristano |
| author_facet | José Yareta-Yareta Marco Galarza Silvia Capristano Oscar Pellón-Cárdenas César Sánchez Jorge Ballon Heinner Guio |
| author_sort | José Yareta-Yareta |
| collection | Producción científica |
| description | OBJECTIVES: To analyze the differential expression of miR-21, miR-29a, miR-99b and miR-155 in serum samples from patients with latent tuberculosis (TB) and active TB compared to healthy controls. MATE RIALS AND METHODS: We used 28 serum samples (9 with active TB, 10 with latent TB and 9 healthy con trols) for the analysis of gene expression by RT-qPCR with Primers and TaqMan probes. The differential expression was calculated by the Livak method using a normalizing gene (RNU-48). RESULTS: Overex pression of miR-155 was found in people with latent tuberculosis, compared to healthy controls (0.63 vs. 0.01; p value = 0.032). CONCLUSION: The miR-155 could be considered a biomarker to differentiate latent TB from active disease. Studies with larger sample sizes are required to corroborate the findings. |
| format | Article |
| id | oai:cris.upeu.edu.pe:20.500.15037/1198 |
| institution | UPeU |
| language | Spanish |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| record_format | dspace |
| spelling | oai:cris.upeu.edu.pe:20.500.15037/11982026-10-30T16:15:21ZExpresión diferencial de micro-ARN circulantes en pacientes con tuberculosis activa y latenteJosé Yareta-YaretaMarco GalarzaSilvia CapristanoOscar Pellón-CárdenasCésar SánchezJorge BallonHeinner GuioLatent tuberculosisMolecular biologyMycobacterium tuberculosisMedicineTuberculosisBiologyPathologyLatent tuberculosisMolecular biologyMycobacterium tuberculosisMedicineTuberculosisBiologyPathologyHumansHumansHumansTuberculosis bloodTuberculosis bloodTuberculosis bloodTuberculosis therapyTuberculosis therapyTuberculosis therapyBiomarkers bloodBiomarkers bloodBiomarkers bloodGene ExpressionGene ExpressionGene ExpressionCase-Control StudiesCase-Control StudiesCase-Control StudiesMicroRNAs bloodMicroRNAs bloodMicroRNAs bloodMicroRNAs geneticsMicroRNAs geneticsMicroRNAs geneticsLatent Tuberculosis bloodLatent Tuberculosis bloodLatent Tuberculosis bloodLatent Tuberculosis therapyLatent Tuberculosis therapyLatent Tuberculosis therapyLife Sciences Biochemistry, Genetics and Molecular Biology Cancer Research MicroRNA in disease regulationHealth Sciences Medicine Infectious Diseases Tuberculosis Research and EpidemiologyHealth Sciences Medicine Surgery Diagnosis and treatment of tuberculosisOBJECTIVES: To analyze the differential expression of miR-21, miR-29a, miR-99b and miR-155 in serum samples from patients with latent tuberculosis (TB) and active TB compared to healthy controls. MATE RIALS AND METHODS: We used 28 serum samples (9 with active TB, 10 with latent TB and 9 healthy con trols) for the analysis of gene expression by RT-qPCR with Primers and TaqMan probes. The differential expression was calculated by the Livak method using a normalizing gene (RNU-48). RESULTS: Overex pression of miR-155 was found in people with latent tuberculosis, compared to healthy controls (0.63 vs. 0.01; p value = 0.032). CONCLUSION: The miR-155 could be considered a biomarker to differentiate latent TB from active disease. Studies with larger sample sizes are required to corroborate the findings.Articlehttps://pubmed.ncbi.nlm.nih.gov/32520192https://rpmesp.ins.gob.pe/rpmesp/article/download/4468/3520https://doi.org/10.17843/rpmesp.2020.371.446810.17843/rpmesp.2020.371.4468https://hdl.handle.net/20.500.15037/1198W3022560670esRevista Peruana de Medicina Experimental y Salud Pública1726-46341726-46422020-03-242026-06-13T09:49:50Z2026-06-13T09:49:50Z |
| spellingShingle | Latent tuberculosis Molecular biology Mycobacterium tuberculosis Medicine Tuberculosis Biology Pathology Latent tuberculosis Molecular biology Mycobacterium tuberculosis Medicine Tuberculosis Biology Pathology Humans Humans Humans Tuberculosis blood Tuberculosis blood Tuberculosis blood Tuberculosis therapy Tuberculosis therapy Tuberculosis therapy Biomarkers blood Biomarkers blood Biomarkers blood Gene Expression Gene Expression Gene Expression Case-Control Studies Case-Control Studies Case-Control Studies MicroRNAs blood MicroRNAs blood MicroRNAs blood MicroRNAs genetics MicroRNAs genetics MicroRNAs genetics Latent Tuberculosis blood Latent Tuberculosis blood Latent Tuberculosis blood Latent Tuberculosis therapy Latent Tuberculosis therapy Latent Tuberculosis therapy Life Sciences Biochemistry, Genetics and Molecular Biology Cancer Research MicroRNA in disease regulation Health Sciences Medicine Infectious Diseases Tuberculosis Research and Epidemiology Health Sciences Medicine Surgery Diagnosis and treatment of tuberculosis José Yareta-Yareta Marco Galarza Silvia Capristano Oscar Pellón-Cárdenas César Sánchez Jorge Ballon Heinner Guio Expresión diferencial de micro-ARN circulantes en pacientes con tuberculosis activa y latente |
| title | Expresión diferencial de micro-ARN circulantes en pacientes con tuberculosis activa y latente |
| title_full | Expresión diferencial de micro-ARN circulantes en pacientes con tuberculosis activa y latente |
| title_fullStr | Expresión diferencial de micro-ARN circulantes en pacientes con tuberculosis activa y latente |
| title_full_unstemmed | Expresión diferencial de micro-ARN circulantes en pacientes con tuberculosis activa y latente |
| title_short | Expresión diferencial de micro-ARN circulantes en pacientes con tuberculosis activa y latente |
| title_sort | expresion diferencial de micro arn circulantes en pacientes con tuberculosis activa y latente |
| topic | Latent tuberculosis Molecular biology Mycobacterium tuberculosis Medicine Tuberculosis Biology Pathology Latent tuberculosis Molecular biology Mycobacterium tuberculosis Medicine Tuberculosis Biology Pathology Humans Humans Humans Tuberculosis blood Tuberculosis blood Tuberculosis blood Tuberculosis therapy Tuberculosis therapy Tuberculosis therapy Biomarkers blood Biomarkers blood Biomarkers blood Gene Expression Gene Expression Gene Expression Case-Control Studies Case-Control Studies Case-Control Studies MicroRNAs blood MicroRNAs blood MicroRNAs blood MicroRNAs genetics MicroRNAs genetics MicroRNAs genetics Latent Tuberculosis blood Latent Tuberculosis blood Latent Tuberculosis blood Latent Tuberculosis therapy Latent Tuberculosis therapy Latent Tuberculosis therapy Life Sciences Biochemistry, Genetics and Molecular Biology Cancer Research MicroRNA in disease regulation Health Sciences Medicine Infectious Diseases Tuberculosis Research and Epidemiology Health Sciences Medicine Surgery Diagnosis and treatment of tuberculosis |
| topic_facet | Latent tuberculosis Molecular biology Mycobacterium tuberculosis Medicine Tuberculosis Biology Pathology Latent tuberculosis Molecular biology Mycobacterium tuberculosis Medicine Tuberculosis Biology Pathology Humans Humans Humans Tuberculosis blood Tuberculosis blood Tuberculosis blood Tuberculosis therapy Tuberculosis therapy Tuberculosis therapy Biomarkers blood Biomarkers blood Biomarkers blood Gene Expression Gene Expression Gene Expression Case-Control Studies Case-Control Studies Case-Control Studies MicroRNAs blood MicroRNAs blood MicroRNAs blood MicroRNAs genetics MicroRNAs genetics MicroRNAs genetics Latent Tuberculosis blood Latent Tuberculosis blood Latent Tuberculosis blood Latent Tuberculosis therapy Latent Tuberculosis therapy Latent Tuberculosis therapy Life Sciences Biochemistry, Genetics and Molecular Biology Cancer Research MicroRNA in disease regulation Health Sciences Medicine Infectious Diseases Tuberculosis Research and Epidemiology Health Sciences Medicine Surgery Diagnosis and treatment of tuberculosis |
| url | https://pubmed.ncbi.nlm.nih.gov/32520192 https://rpmesp.ins.gob.pe/rpmesp/article/download/4468/3520 https://doi.org/10.17843/rpmesp.2020.371.4468 https://hdl.handle.net/20.500.15037/1198 |
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