Genes resistentes a carbapenémicos y colistina aislados en Musca domestica proveniente de un basural cercano a un hospital de Lima
Motivation for the study. The presence of antibiotic resistance genes in bacteria isolated from common flies is a potential public health hazard because it facilitates the presence and spread of antibiotic resistance genes in the environment. Main findings. Thirty-eight bacterial strains identified...
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2024
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| author | Alarcón-Calle, Miguel A. Osorio-Guevara, Víctor L. Salas-Asencios, Ramsés Yareta Yareta, José Luis Marcos Carbajal, Pool Rodrigo-Rojas, María E. |
| author_browse | Alarcón-Calle, Miguel A. Marcos Carbajal, Pool Osorio-Guevara, Víctor L. Rodrigo-Rojas, María E. Salas-Asencios, Ramsés Yareta Yareta, José Luis |
| author_facet | Alarcón-Calle, Miguel A. Osorio-Guevara, Víctor L. Salas-Asencios, Ramsés Yareta Yareta, José Luis Marcos Carbajal, Pool Rodrigo-Rojas, María E. |
| author_sort | Alarcón-Calle, Miguel A. |
| collection | Producción científica |
| description | Motivation for the study. The presence of antibiotic resistance genes in bacteria isolated from common flies is a potential public health hazard because it facilitates the presence and spread of antibiotic resistance genes in the environment. Main findings. Thirty-eight bacterial strains identified in 14 species were isolated from within the fly bodies, of which 31 strains showed resistance to carbapenems and 26 strains showed resistance to colistin. Seven bacterial strains showed carbapenem resistance genes and one Escherichia coli strain had resistance to KPC, OXA-48 and mcr-1. Implications. This is the first report of antibiotic resistance genes in bacteria carried by common flies in Peru. The objective was to determine the presence of carbapenem resistance genes and plasmid resistance to colistin (mcr-1) in bacteria isolated from Musca domestica in a garbage dump near a hospital in Lima, Peru. Bacteria with phenotypic resistance to carbapenemics were isolated on CHROMagar mSuperCARBATM medium and colistin resistance profiling was performed using the colistin disk elution method. Detection of blaKPC, blaNDM, blaIMP, blaOXA-48, blaVIM and mcr-1 genes was performed by conventional PCR. The antimicrobial susceptibility profile was determined using the automated MicroScan system. We found that 31/38 strains had phenotypic resistance to carbapenemics and 26/38 strains had phenotypic resistance to colistin with a minimum inhibitory concentration ≥ 4 µg/ml. Finally, we identified seven bacterial strains with carbapenem resistance genes (OXA-48 and KPC) and one bacterial strain with plasmid resistance to colistin (mcr-1). One Escherichia coli strain had three resistance genes: KPC, OXA-48 and mcr-1. |
| format | Article |
| id | oai:cris.upeu.edu.pe:20.500.15037/1134 |
| institution | UPeU |
| language | English |
| publishDate | 2024 |
| publishDateRange | 2024 |
| publishDateSort | 2024 |
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| spelling | oai:cris.upeu.edu.pe:20.500.15037/11342026-11-02T12:57:04ZGenes resistentes a carbapenémicos y colistina aislados en Musca domestica proveniente de un basural cercano a un hospital de LimaAlarcón-Calle, Miguel A.Osorio-Guevara, Víctor L.Salas-Asencios, RamsésYareta Yareta, José LuisMarcos Carbajal, PoolRodrigo-Rojas, María E.moscas domésticasfarmacorresistencia microbianacolistinacarbapenémicosMotivation for the study. The presence of antibiotic resistance genes in bacteria isolated from common flies is a potential public health hazard because it facilitates the presence and spread of antibiotic resistance genes in the environment. Main findings. Thirty-eight bacterial strains identified in 14 species were isolated from within the fly bodies, of which 31 strains showed resistance to carbapenems and 26 strains showed resistance to colistin. Seven bacterial strains showed carbapenem resistance genes and one Escherichia coli strain had resistance to KPC, OXA-48 and mcr-1. Implications. This is the first report of antibiotic resistance genes in bacteria carried by common flies in Peru. The objective was to determine the presence of carbapenem resistance genes and plasmid resistance to colistin (mcr-1) in bacteria isolated from Musca domestica in a garbage dump near a hospital in Lima, Peru. Bacteria with phenotypic resistance to carbapenemics were isolated on CHROMagar mSuperCARBATM medium and colistin resistance profiling was performed using the colistin disk elution method. Detection of blaKPC, blaNDM, blaIMP, blaOXA-48, blaVIM and mcr-1 genes was performed by conventional PCR. The antimicrobial susceptibility profile was determined using the automated MicroScan system. We found that 31/38 strains had phenotypic resistance to carbapenemics and 26/38 strains had phenotypic resistance to colistin with a minimum inhibitory concentration ≥ 4 µg/ml. Finally, we identified seven bacterial strains with carbapenem resistance genes (OXA-48 and KPC) and one bacterial strain with plasmid resistance to colistin (mcr-1). One Escherichia coli strain had three resistance genes: KPC, OXA-48 and mcr-1.Articlehttps://pubmed.ncbi.nlm.nih.gov/39166639https://rpmesp.ins.gob.pe/index.php/rpmesp/article/download/13257/5622https://doi.org/10.17843/rpmesp.2024.412.13257PMC1130068210.17843/rpmesp.2024.412.13257https://hdl.handle.net/20.500.15037/113439166639W4399879841enRevista Peruana de Medicina Experimental y Salud Pública1726-46341726-46422024-06-212026-06-13T09:47:15Z2026-06-13T09:47:15Z |
| spellingShingle | moscas domésticas farmacorresistencia microbiana colistina carbapenémicos Alarcón-Calle, Miguel A. Osorio-Guevara, Víctor L. Salas-Asencios, Ramsés Yareta Yareta, José Luis Marcos Carbajal, Pool Rodrigo-Rojas, María E. Genes resistentes a carbapenémicos y colistina aislados en Musca domestica proveniente de un basural cercano a un hospital de Lima |
| title | Genes resistentes a carbapenémicos y colistina aislados en Musca domestica proveniente de un basural cercano a un hospital de Lima |
| title_full | Genes resistentes a carbapenémicos y colistina aislados en Musca domestica proveniente de un basural cercano a un hospital de Lima |
| title_fullStr | Genes resistentes a carbapenémicos y colistina aislados en Musca domestica proveniente de un basural cercano a un hospital de Lima |
| title_full_unstemmed | Genes resistentes a carbapenémicos y colistina aislados en Musca domestica proveniente de un basural cercano a un hospital de Lima |
| title_short | Genes resistentes a carbapenémicos y colistina aislados en Musca domestica proveniente de un basural cercano a un hospital de Lima |
| title_sort | genes resistentes a carbapenemicos y colistina aislados en musca domestica proveniente de un basural cercano a un hospital de lima |
| topic | moscas domésticas farmacorresistencia microbiana colistina carbapenémicos |
| topic_facet | moscas domésticas farmacorresistencia microbiana colistina carbapenémicos |
| url | https://pubmed.ncbi.nlm.nih.gov/39166639 https://rpmesp.ins.gob.pe/index.php/rpmesp/article/download/13257/5622 https://doi.org/10.17843/rpmesp.2024.412.13257 https://hdl.handle.net/20.500.15037/1134 |
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