Diversidad genómica de Escherichia coli uropatógena en aislados clínicos de seis países de Latinoamérica, 2018-2023
BACKGROUND: Motivation for the study. To contribute to the genomic surveillance of UPEC in clinical samples from Latin America, in response to the growing public health problem represented by UTIs and their resistance to antimicrobials. Main findings. Our study revealed a high frequency of high-risk...
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2025
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| author | Caballero, Francesca Martinez-Ventura, Anne Cuicapuza Arteaga, Diego Fajardo-Loyola, Alex Gutierrez-Ajalcriña, Rosmery Soto-Pastrana, Javier Asmat-Marrufo, Percy Barco-Yaipen de Vera, Evelyn Meza-Fernandez, Henry Chambi-Quispe, Mario Pino-Dueñas, Jimena Laura-Rivas, Nicomedes Briones-Alejo, Alexander Diaz-Rengifo, Pilar Peralta-Siesquen, Carlos Salvatierra, Guillermo Tsukayama Cisneros, Pablo Marcos Carbajal, Pool |
| author_browse | Asmat-Marrufo, Percy Barco-Yaipen de Vera, Evelyn Briones-Alejo, Alexander Caballero, Francesca Chambi-Quispe, Mario Cuicapuza Arteaga, Diego Diaz-Rengifo, Pilar Fajardo-Loyola, Alex Gutierrez-Ajalcriña, Rosmery Laura-Rivas, Nicomedes Marcos Carbajal, Pool Martinez-Ventura, Anne Meza-Fernandez, Henry Peralta-Siesquen, Carlos Pino-Dueñas, Jimena Salvatierra, Guillermo Soto-Pastrana, Javier Tsukayama Cisneros, Pablo |
| author_facet | Caballero, Francesca Martinez-Ventura, Anne Cuicapuza Arteaga, Diego Fajardo-Loyola, Alex Gutierrez-Ajalcriña, Rosmery Soto-Pastrana, Javier Asmat-Marrufo, Percy Barco-Yaipen de Vera, Evelyn Meza-Fernandez, Henry Chambi-Quispe, Mario Pino-Dueñas, Jimena Laura-Rivas, Nicomedes Briones-Alejo, Alexander Diaz-Rengifo, Pilar Peralta-Siesquen, Carlos Salvatierra, Guillermo Tsukayama Cisneros, Pablo Marcos Carbajal, Pool |
| author_sort | Caballero, Francesca |
| collection | Producción científica |
| description | BACKGROUND: Motivation for the study. To contribute to the genomic surveillance of UPEC in clinical samples from Latin America, in response to the growing public health problem represented by UTIs and their resistance to antimicrobials. Main findings. Our study revealed a high frequency of high-risk clones, such as ST131 and ST1193. Critical mutations were identified in genes associated with resistance to multiple antibiotics, including fluoroquinolones, beta-lactams, and fosfomycin. Implications. Our results highlight the urgent need to strengthen UPEC surveillance in Latin America. Tracking resistant strains and implementing measures to limit their spread is crucial and has a significant impact on the effectiveness of available treatments. OBJECTIVE.: To genetically characterize clinical isolates of uropathogenic Escherichia coli (UPEC) from hospitals in Peru and contextualize them against 127 additional UPEC genomes reported in six Latin American countries between 2018 and 2023. MATERIALS AND METHODS.: The genomes of 16 Peruvian UPEC isolates were sequenced, assembled and supplemented with 127 genomes available in the NCBI public database. Serotypes, sequence types (STs), antimicrobial resistance (AMR) genes, and resistance-associated mutations were identified. A phylogenetic analysis was also conducted in order to determine evolutionary relations and distribution in phylogroups. RESULTS.: The ST131 clone was the most prevalent (42.7%), followed by ST1193 (13.3%). Phylogroup B2 was widely predominant (83.2%), with serotype O25:H4 standing out. The resistance genes blaTEM-1, blaCTX-M-15, and blaCTX-M-27 were identified with high frequency, as well as mutations in gyrA and parC associated with fluoroquinolone resistance, especially in the ST131 clone. CONCLUSION.: Our findings show high circulation of high-risk UPEC clones, such as ST131 and ST1193, in Latin America, along with a notable burden of genes and mutations linked to multidrug resistance, highlighting the need to strengthen regional genomic surveillance. BACKGROUND: Motivation for the study. To contribute to the genomic surveillance of UPEC in clinical samples from Latin America, in response to the growing public health problem represented by UTIs and their resistance to antimicrobials. Main findings. Our study revealed a high frequency of high-risk clones, such as ST131 and ST1193. Critical mutations were identified in genes associated with resistance to multiple antibiotics, including fluoroquinolones, beta-lactams, and fosfomycin. Implications. Our results highlight the urgent need to strengthen UPEC surveillance in Latin America. Tracking resistant strains and implementing measures to limit their spread is crucial and has a significant impact on the effectiveness of available treatments. |
| format | Article |
| id | oai:cris.upeu.edu.pe:20.500.15037/1129 |
| institution | UPeU |
| language | English |
| publishDate | 2025 |
| publishDateRange | 2025 |
| publishDateSort | 2025 |
| record_format | dspace |
| spelling | oai:cris.upeu.edu.pe:20.500.15037/11292026-11-02T22:00:04ZDiversidad genómica de Escherichia coli uropatógena en aislados clínicos de seis países de Latinoamérica, 2018-2023Caballero, FrancescaMartinez-Ventura, AnneCuicapuza Arteaga, DiegoFajardo-Loyola, AlexGutierrez-Ajalcriña, RosmerySoto-Pastrana, JavierAsmat-Marrufo, PercyBarco-Yaipen de Vera, EvelynMeza-Fernandez, HenryChambi-Quispe, MarioPino-Dueñas, JimenaLaura-Rivas, NicomedesBriones-Alejo, AlexanderDiaz-Rengifo, PilarPeralta-Siesquen, CarlosSalvatierra, GuillermoTsukayama Cisneros, PabloMarcos Carbajal, PoolBACKGROUND: Motivation for the study. To contribute to the genomic surveillance of UPEC in clinical samples from Latin America, in response to the growing public health problem represented by UTIs and their resistance to antimicrobials. Main findings. Our study revealed a high frequency of high-risk clones, such as ST131 and ST1193. Critical mutations were identified in genes associated with resistance to multiple antibiotics, including fluoroquinolones, beta-lactams, and fosfomycin. Implications. Our results highlight the urgent need to strengthen UPEC surveillance in Latin America. Tracking resistant strains and implementing measures to limit their spread is crucial and has a significant impact on the effectiveness of available treatments. OBJECTIVE.: To genetically characterize clinical isolates of uropathogenic Escherichia coli (UPEC) from hospitals in Peru and contextualize them against 127 additional UPEC genomes reported in six Latin American countries between 2018 and 2023. MATERIALS AND METHODS.: The genomes of 16 Peruvian UPEC isolates were sequenced, assembled and supplemented with 127 genomes available in the NCBI public database. Serotypes, sequence types (STs), antimicrobial resistance (AMR) genes, and resistance-associated mutations were identified. A phylogenetic analysis was also conducted in order to determine evolutionary relations and distribution in phylogroups. RESULTS.: The ST131 clone was the most prevalent (42.7%), followed by ST1193 (13.3%). Phylogroup B2 was widely predominant (83.2%), with serotype O25:H4 standing out. The resistance genes blaTEM-1, blaCTX-M-15, and blaCTX-M-27 were identified with high frequency, as well as mutations in gyrA and parC associated with fluoroquinolone resistance, especially in the ST131 clone. CONCLUSION.: Our findings show high circulation of high-risk UPEC clones, such as ST131 and ST1193, in Latin America, along with a notable burden of genes and mutations linked to multidrug resistance, highlighting the need to strengthen regional genomic surveillance. BACKGROUND: Motivation for the study. To contribute to the genomic surveillance of UPEC in clinical samples from Latin America, in response to the growing public health problem represented by UTIs and their resistance to antimicrobials. Main findings. Our study revealed a high frequency of high-risk clones, such as ST131 and ST1193. Critical mutations were identified in genes associated with resistance to multiple antibiotics, including fluoroquinolones, beta-lactams, and fosfomycin. Implications. Our results highlight the urgent need to strengthen UPEC surveillance in Latin America. Tracking resistant strains and implementing measures to limit their spread is crucial and has a significant impact on the effectiveness of available treatments.Articlehttps://pubmed.ncbi.nlm.nih.gov/40900482https://doi.org/10.17843/rpmesp.2025.422.14299PMC1237789110.17843/rpmesp.2025.422.14299https://hdl.handle.net/20.500.15037/112940900482W4413970267enRevista Peruana de Medicina Experimental y Salud Pública1726-46341726-46422025-06-192026-06-13T09:47:06Z2026-06-13T09:47:06Z |
| spellingShingle | Caballero, Francesca Martinez-Ventura, Anne Cuicapuza Arteaga, Diego Fajardo-Loyola, Alex Gutierrez-Ajalcriña, Rosmery Soto-Pastrana, Javier Asmat-Marrufo, Percy Barco-Yaipen de Vera, Evelyn Meza-Fernandez, Henry Chambi-Quispe, Mario Pino-Dueñas, Jimena Laura-Rivas, Nicomedes Briones-Alejo, Alexander Diaz-Rengifo, Pilar Peralta-Siesquen, Carlos Salvatierra, Guillermo Tsukayama Cisneros, Pablo Marcos Carbajal, Pool Diversidad genómica de Escherichia coli uropatógena en aislados clínicos de seis países de Latinoamérica, 2018-2023 |
| title | Diversidad genómica de Escherichia coli uropatógena en aislados clínicos de seis países de Latinoamérica, 2018-2023 |
| title_full | Diversidad genómica de Escherichia coli uropatógena en aislados clínicos de seis países de Latinoamérica, 2018-2023 |
| title_fullStr | Diversidad genómica de Escherichia coli uropatógena en aislados clínicos de seis países de Latinoamérica, 2018-2023 |
| title_full_unstemmed | Diversidad genómica de Escherichia coli uropatógena en aislados clínicos de seis países de Latinoamérica, 2018-2023 |
| title_short | Diversidad genómica de Escherichia coli uropatógena en aislados clínicos de seis países de Latinoamérica, 2018-2023 |
| title_sort | diversidad genomica de escherichia coli uropatogena en aislados clinicos de seis paises de latinoamerica 2018 2023 |
| url | https://pubmed.ncbi.nlm.nih.gov/40900482 https://doi.org/10.17843/rpmesp.2025.422.14299 https://hdl.handle.net/20.500.15037/1129 |
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