Emergence of carbapenem-resistant Pseudomonas aeruginosa ST179 producing both IMP-16 and KPC-2: a case study of introduction from Peru to Spain
ABSTRACT We describe four cases of a novel carbapenem-resistant Pseudomonas aeruginosa ST179 clone carrying the bla KPC-2 or bla KPC-35 gene together with bla IMP-16 , imported from Peru to Spain and isolated from leukemia patients. All isolates were multidrug-resistant but remained susceptible to f...
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2024
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| author | Joaquim Viñes Carlos Lopera Andrea Vergara Ignasi Roca Jordi Vilà Climent Casals‐Pascual José Antonio Martínez Carolina García‐Vidal Àlex Soriano Cristina Pitart |
| author_browse | Andrea Vergara Carlos Lopera Carolina García‐Vidal Climent Casals‐Pascual Cristina Pitart Ignasi Roca Joaquim Viñes Jordi Vilà José Antonio Martínez Àlex Soriano |
| author_facet | Joaquim Viñes Carlos Lopera Andrea Vergara Ignasi Roca Jordi Vilà Climent Casals‐Pascual José Antonio Martínez Carolina García‐Vidal Àlex Soriano Cristina Pitart |
| author_sort | Joaquim Viñes |
| collection | Producción científica |
| description | ABSTRACT We describe four cases of a novel carbapenem-resistant Pseudomonas aeruginosa ST179 clone carrying the bla KPC-2 or bla KPC-35 gene together with bla IMP-16 , imported from Peru to Spain and isolated from leukemia patients. All isolates were multidrug-resistant but remained susceptible to fosfomycin, cefiderocol, and colistin. Whole-genome sequencing revealed that bla KPC-2 and bla KPC-35 were located in an IncP6 plasmid, whereas bla IMP-16 was in a chromosomal type 1 integron. This study highlights the global threat of multidrug-resistant P. aeruginosa clones and underscores the importance of monitoring and early detection of emerging resistance mechanisms to guide appropriate treatment strategies. The importation and spread of such clones emphasize the urgent need to implement strict infection control measures to prevent the dissemination of carbapenem-resistant bacteria. IMPORTANCE This is the first documented case of a Pseudomonas aeruginosa ST179 strain carrying the blaKPC-35 gene, and it represents the first report of a P. aeruginosa co-harboring blaIMP-16 and either blaKPC-2 or blaKPC-35, which wre imported from Peru to Spain, highlighting a threat due to the capacity of spreading carbapenem-resistance via plasmid conjugation. |
| format | Article |
| id | oai:cris.upeu.edu.pe:20.500.15037/1052 |
| institution | UPeU |
| language | English |
| publishDate | 2024 |
| publishDateRange | 2024 |
| publishDateSort | 2024 |
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| spelling | oai:cris.upeu.edu.pe:20.500.15037/10522026-10-30T16:15:08ZEmergence of carbapenem-resistant Pseudomonas aeruginosa ST179 producing both IMP-16 and KPC-2: a case study of introduction from Peru to SpainJoaquim ViñesCarlos LoperaAndrea VergaraIgnasi RocaJordi VilàCliment Casals‐PascualJosé Antonio MartínezCarolina García‐VidalÀlex SorianoCristina PitartPseudomonas aeruginosaCarbapenemMicrobiologyBiologyBacteriaGeneticsAntibioticsPseudomonas aeruginosaCarbapenemMicrobiologyBiologyBacteriaGeneticsAntibioticsWhole Genome SequencingWhole Genome SequencingWhole Genome SequencingWhole Genome SequencingWhole Genome SequencingAdultAdultAdultAdultAdultAnti-Bacterial Agents pharmacologyAnti-Bacterial Agents pharmacologyAnti-Bacterial Agents pharmacologyAnti-Bacterial Agents pharmacologyAnti-Bacterial Agents pharmacologyBacterial Proteins geneticsBacterial Proteins geneticsBacterial Proteins geneticsBacterial Proteins geneticsBacterial Proteins geneticsBacterial Proteins metabolismBacterial Proteins metabolismBacterial Proteins metabolismBacterial Proteins metabolismBacterial Proteins metabolismbeta-Lactamases geneticsbeta-Lactamases geneticsbeta-Lactamases geneticsbeta-Lactamases geneticsbeta-Lactamases geneticsbeta-Lactamases metabolismbeta-Lactamases metabolismbeta-Lactamases metabolismbeta-Lactamases metabolismbeta-Lactamases metabolismFemaleFemaleFemaleFemaleFemaleHumansHumansHumansHumansHumansMaleMaleMaleMaleMaleMicrobial Sensitivity TestsMicrobial Sensitivity TestsMicrobial Sensitivity TestsMicrobial Sensitivity TestsMicrobial Sensitivity TestsMiddle AgedMiddle AgedMiddle AgedMiddle AgedMiddle AgedPeruPeruPeruPeruPeruPlasmids geneticsPlasmids geneticsPlasmids geneticsPlasmids geneticsPlasmids geneticsPseudomonas aeruginosa drug effectsPseudomonas aeruginosa drug effectsPseudomonas aeruginosa drug effectsPseudomonas aeruginosa drug effectsPseudomonas aeruginosa drug effectsPseudomonas aeruginosa enzymologyPseudomonas aeruginosa enzymologyPseudomonas aeruginosa enzymologyPseudomonas aeruginosa enzymologyPseudomonas aeruginosa enzymologyPseudomonas aeruginosa geneticsPseudomonas aeruginosa geneticsPseudomonas aeruginosa geneticsPseudomonas aeruginosa geneticsPseudomonas aeruginosa geneticsPseudomonas aeruginosa isolation & purificationPseudomonas aeruginosa isolation & purificationPseudomonas aeruginosa isolation & purificationPseudomonas aeruginosa isolation & purificationPseudomonas aeruginosa isolation & purificationPseudomonas Infections microbiologyPseudomonas Infections microbiologyPseudomonas Infections microbiologyPseudomonas Infections microbiologyPseudomonas Infections microbiologySpainSpainSpainSpainSpainCarbapenems pharmacologyCarbapenems pharmacologyCarbapenems pharmacologyCarbapenems pharmacologyCarbapenems pharmacologyDrug Resistance, Multiple, Bacterial geneticsDrug Resistance, Multiple, Bacterial geneticsDrug Resistance, Multiple, Bacterial geneticsDrug Resistance, Multiple, Bacterial geneticsDrug Resistance, Multiple, Bacterial geneticsLife Sciences Biochemistry, Genetics and Molecular Biology Molecular Medicine Antibiotic Resistance in BacteriaLife Sciences Immunology and Microbiology Applied Microbiology and Biotechnology Antibiotic Use and ResistanceLife Sciences Biochemistry, Genetics and Molecular Biology Molecular Biology Bacterial biofilms and quorum sensingABSTRACT We describe four cases of a novel carbapenem-resistant Pseudomonas aeruginosa ST179 clone carrying the bla KPC-2 or bla KPC-35 gene together with bla IMP-16 , imported from Peru to Spain and isolated from leukemia patients. All isolates were multidrug-resistant but remained susceptible to fosfomycin, cefiderocol, and colistin. Whole-genome sequencing revealed that bla KPC-2 and bla KPC-35 were located in an IncP6 plasmid, whereas bla IMP-16 was in a chromosomal type 1 integron. This study highlights the global threat of multidrug-resistant P. aeruginosa clones and underscores the importance of monitoring and early detection of emerging resistance mechanisms to guide appropriate treatment strategies. The importation and spread of such clones emphasize the urgent need to implement strict infection control measures to prevent the dissemination of carbapenem-resistant bacteria. IMPORTANCE This is the first documented case of a Pseudomonas aeruginosa ST179 strain carrying the blaKPC-35 gene, and it represents the first report of a P. aeruginosa co-harboring blaIMP-16 and either blaKPC-2 or blaKPC-35, which wre imported from Peru to Spain, highlighting a threat due to the capacity of spreading carbapenem-resistance via plasmid conjugation.Articlehttps://pubmed.ncbi.nlm.nih.gov/38727230https://doi.org/10.1128/spectrum.00614-2410.1128/spectrum.00614-24https://hdl.handle.net/20.500.15037/1052W4396799314enMicrobiology Spectrum2165-04972024-05-102026-06-13T09:44:45Z2026-06-13T09:44:45Z |
| spellingShingle | Pseudomonas aeruginosa Carbapenem Microbiology Biology Bacteria Genetics Antibiotics Pseudomonas aeruginosa Carbapenem Microbiology Biology Bacteria Genetics Antibiotics Whole Genome Sequencing Whole Genome Sequencing Whole Genome Sequencing Whole Genome Sequencing Whole Genome Sequencing Adult Adult Adult Adult Adult Anti-Bacterial Agents pharmacology Anti-Bacterial Agents pharmacology Anti-Bacterial Agents pharmacology Anti-Bacterial Agents pharmacology Anti-Bacterial Agents pharmacology Bacterial Proteins genetics Bacterial Proteins genetics Bacterial Proteins genetics Bacterial Proteins genetics Bacterial Proteins genetics Bacterial Proteins metabolism Bacterial Proteins metabolism Bacterial Proteins metabolism Bacterial Proteins metabolism Bacterial Proteins metabolism beta-Lactamases genetics beta-Lactamases genetics beta-Lactamases genetics beta-Lactamases genetics beta-Lactamases genetics beta-Lactamases metabolism beta-Lactamases metabolism beta-Lactamases metabolism beta-Lactamases metabolism beta-Lactamases metabolism Female Female Female Female Female Humans Humans Humans Humans Humans Male Male Male Male Male Microbial Sensitivity Tests Microbial Sensitivity Tests Microbial Sensitivity Tests Microbial Sensitivity Tests Microbial Sensitivity Tests Middle Aged Middle Aged Middle Aged Middle Aged Middle Aged Peru Peru Peru Peru Peru Plasmids genetics Plasmids genetics Plasmids genetics Plasmids genetics Plasmids genetics Pseudomonas aeruginosa drug effects Pseudomonas aeruginosa drug effects Pseudomonas aeruginosa drug effects Pseudomonas aeruginosa drug effects Pseudomonas aeruginosa drug effects Pseudomonas aeruginosa enzymology Pseudomonas aeruginosa enzymology Pseudomonas aeruginosa enzymology Pseudomonas aeruginosa enzymology Pseudomonas aeruginosa enzymology Pseudomonas aeruginosa genetics Pseudomonas aeruginosa genetics Pseudomonas aeruginosa genetics Pseudomonas aeruginosa genetics Pseudomonas aeruginosa genetics Pseudomonas aeruginosa isolation & purification Pseudomonas aeruginosa isolation & purification Pseudomonas aeruginosa isolation & purification Pseudomonas aeruginosa isolation & purification Pseudomonas aeruginosa isolation & purification Pseudomonas Infections microbiology Pseudomonas Infections microbiology Pseudomonas Infections microbiology Pseudomonas Infections microbiology Pseudomonas Infections microbiology Spain Spain Spain Spain Spain Carbapenems pharmacology Carbapenems pharmacology Carbapenems pharmacology Carbapenems pharmacology Carbapenems pharmacology Drug Resistance, Multiple, Bacterial genetics Drug Resistance, Multiple, Bacterial genetics Drug Resistance, Multiple, Bacterial genetics Drug Resistance, Multiple, Bacterial genetics Drug Resistance, Multiple, Bacterial genetics Life Sciences Biochemistry, Genetics and Molecular Biology Molecular Medicine Antibiotic Resistance in Bacteria Life Sciences Immunology and Microbiology Applied Microbiology and Biotechnology Antibiotic Use and Resistance Life Sciences Biochemistry, Genetics and Molecular Biology Molecular Biology Bacterial biofilms and quorum sensing Joaquim Viñes Carlos Lopera Andrea Vergara Ignasi Roca Jordi Vilà Climent Casals‐Pascual José Antonio Martínez Carolina García‐Vidal Àlex Soriano Cristina Pitart Emergence of carbapenem-resistant Pseudomonas aeruginosa ST179 producing both IMP-16 and KPC-2: a case study of introduction from Peru to Spain |
| title | Emergence of carbapenem-resistant Pseudomonas aeruginosa ST179 producing both IMP-16 and KPC-2: a case study of introduction from Peru to Spain |
| title_full | Emergence of carbapenem-resistant Pseudomonas aeruginosa ST179 producing both IMP-16 and KPC-2: a case study of introduction from Peru to Spain |
| title_fullStr | Emergence of carbapenem-resistant Pseudomonas aeruginosa ST179 producing both IMP-16 and KPC-2: a case study of introduction from Peru to Spain |
| title_full_unstemmed | Emergence of carbapenem-resistant Pseudomonas aeruginosa ST179 producing both IMP-16 and KPC-2: a case study of introduction from Peru to Spain |
| title_short | Emergence of carbapenem-resistant Pseudomonas aeruginosa ST179 producing both IMP-16 and KPC-2: a case study of introduction from Peru to Spain |
| title_sort | emergence of carbapenem resistant pseudomonas aeruginosa st179 producing both imp 16 and kpc 2 a case study of introduction from peru to spain |
| topic | Pseudomonas aeruginosa Carbapenem Microbiology Biology Bacteria Genetics Antibiotics Pseudomonas aeruginosa Carbapenem Microbiology Biology Bacteria Genetics Antibiotics Whole Genome Sequencing Whole Genome Sequencing Whole Genome Sequencing Whole Genome Sequencing Whole Genome Sequencing Adult Adult Adult Adult Adult Anti-Bacterial Agents pharmacology Anti-Bacterial Agents pharmacology Anti-Bacterial Agents pharmacology Anti-Bacterial Agents pharmacology Anti-Bacterial Agents pharmacology Bacterial Proteins genetics Bacterial Proteins genetics Bacterial Proteins genetics Bacterial Proteins genetics Bacterial Proteins genetics Bacterial Proteins metabolism Bacterial Proteins metabolism Bacterial Proteins metabolism Bacterial Proteins metabolism Bacterial Proteins metabolism beta-Lactamases genetics beta-Lactamases genetics beta-Lactamases genetics beta-Lactamases genetics beta-Lactamases genetics beta-Lactamases metabolism beta-Lactamases metabolism beta-Lactamases metabolism beta-Lactamases metabolism beta-Lactamases metabolism Female Female Female Female Female Humans Humans Humans Humans Humans Male Male Male Male Male Microbial Sensitivity Tests Microbial Sensitivity Tests Microbial Sensitivity Tests Microbial Sensitivity Tests Microbial Sensitivity Tests Middle Aged Middle Aged Middle Aged Middle Aged Middle Aged Peru Peru Peru Peru Peru Plasmids genetics Plasmids genetics Plasmids genetics Plasmids genetics Plasmids genetics Pseudomonas aeruginosa drug effects Pseudomonas aeruginosa drug effects Pseudomonas aeruginosa drug effects Pseudomonas aeruginosa drug effects Pseudomonas aeruginosa drug effects Pseudomonas aeruginosa enzymology Pseudomonas aeruginosa enzymology Pseudomonas aeruginosa enzymology Pseudomonas aeruginosa enzymology Pseudomonas aeruginosa enzymology Pseudomonas aeruginosa genetics Pseudomonas aeruginosa genetics Pseudomonas aeruginosa genetics Pseudomonas aeruginosa genetics Pseudomonas aeruginosa genetics Pseudomonas aeruginosa isolation & purification Pseudomonas aeruginosa isolation & purification Pseudomonas aeruginosa isolation & purification Pseudomonas aeruginosa isolation & purification Pseudomonas aeruginosa isolation & purification Pseudomonas Infections microbiology Pseudomonas Infections microbiology Pseudomonas Infections microbiology Pseudomonas Infections microbiology Pseudomonas Infections microbiology Spain Spain Spain Spain Spain Carbapenems pharmacology Carbapenems pharmacology Carbapenems pharmacology Carbapenems pharmacology Carbapenems pharmacology Drug Resistance, Multiple, Bacterial genetics Drug Resistance, Multiple, Bacterial genetics Drug Resistance, Multiple, Bacterial genetics Drug Resistance, Multiple, Bacterial genetics Drug Resistance, Multiple, Bacterial genetics Life Sciences Biochemistry, Genetics and Molecular Biology Molecular Medicine Antibiotic Resistance in Bacteria Life Sciences Immunology and Microbiology Applied Microbiology and Biotechnology Antibiotic Use and Resistance Life Sciences Biochemistry, Genetics and Molecular Biology Molecular Biology Bacterial biofilms and quorum sensing |
| topic_facet | Pseudomonas aeruginosa Carbapenem Microbiology Biology Bacteria Genetics Antibiotics Pseudomonas aeruginosa Carbapenem Microbiology Biology Bacteria Genetics Antibiotics Whole Genome Sequencing Whole Genome Sequencing Whole Genome Sequencing Whole Genome Sequencing Whole Genome Sequencing Adult Adult Adult Adult Adult Anti-Bacterial Agents pharmacology Anti-Bacterial Agents pharmacology Anti-Bacterial Agents pharmacology Anti-Bacterial Agents pharmacology Anti-Bacterial Agents pharmacology Bacterial Proteins genetics Bacterial Proteins genetics Bacterial Proteins genetics Bacterial Proteins genetics Bacterial Proteins genetics Bacterial Proteins metabolism Bacterial Proteins metabolism Bacterial Proteins metabolism Bacterial Proteins metabolism Bacterial Proteins metabolism beta-Lactamases genetics beta-Lactamases genetics beta-Lactamases genetics beta-Lactamases genetics beta-Lactamases genetics beta-Lactamases metabolism beta-Lactamases metabolism beta-Lactamases metabolism beta-Lactamases metabolism beta-Lactamases metabolism Female Female Female Female Female Humans Humans Humans Humans Humans Male Male Male Male Male Microbial Sensitivity Tests Microbial Sensitivity Tests Microbial Sensitivity Tests Microbial Sensitivity Tests Microbial Sensitivity Tests Middle Aged Middle Aged Middle Aged Middle Aged Middle Aged Peru Peru Peru Peru Peru Plasmids genetics Plasmids genetics Plasmids genetics Plasmids genetics Plasmids genetics Pseudomonas aeruginosa drug effects Pseudomonas aeruginosa drug effects Pseudomonas aeruginosa drug effects Pseudomonas aeruginosa drug effects Pseudomonas aeruginosa drug effects Pseudomonas aeruginosa enzymology Pseudomonas aeruginosa enzymology Pseudomonas aeruginosa enzymology Pseudomonas aeruginosa enzymology Pseudomonas aeruginosa enzymology Pseudomonas aeruginosa genetics Pseudomonas aeruginosa genetics Pseudomonas aeruginosa genetics Pseudomonas aeruginosa genetics Pseudomonas aeruginosa genetics Pseudomonas aeruginosa isolation & purification Pseudomonas aeruginosa isolation & purification Pseudomonas aeruginosa isolation & purification Pseudomonas aeruginosa isolation & purification Pseudomonas aeruginosa isolation & purification Pseudomonas Infections microbiology Pseudomonas Infections microbiology Pseudomonas Infections microbiology Pseudomonas Infections microbiology Pseudomonas Infections microbiology Spain Spain Spain Spain Spain Carbapenems pharmacology Carbapenems pharmacology Carbapenems pharmacology Carbapenems pharmacology Carbapenems pharmacology Drug Resistance, Multiple, Bacterial genetics Drug Resistance, Multiple, Bacterial genetics Drug Resistance, Multiple, Bacterial genetics Drug Resistance, Multiple, Bacterial genetics Drug Resistance, Multiple, Bacterial genetics Life Sciences Biochemistry, Genetics and Molecular Biology Molecular Medicine Antibiotic Resistance in Bacteria Life Sciences Immunology and Microbiology Applied Microbiology and Biotechnology Antibiotic Use and Resistance Life Sciences Biochemistry, Genetics and Molecular Biology Molecular Biology Bacterial biofilms and quorum sensing |
| url | https://pubmed.ncbi.nlm.nih.gov/38727230 https://doi.org/10.1128/spectrum.00614-24 https://hdl.handle.net/20.500.15037/1052 |
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